Rpn2 Antibody - BSA Free

Immunohistochemistry;Rpn2 Antibody[NBP3-30572]Detection of mouse PSMD1 by immunohistochemistry. Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti- PSMD1 used at a dilution of 1:5,000 . Detection: DAB

Immunohistochemistry;Rpn2 Antibody[NBP3-30571]Detection of human PSMD1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- PSMD1 used at a dilution of 1:5,000 . Detection: DAB

Immunoprecipitation:Rpn2 AntibodyNBP3-30572] -Detection of human PSMD1 by western blot of immunoprecipitates. Samples: Whole cell lysate (200 µg per IP reaction; 5% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PSMD1 antibody (NBP3-30572 lot 2) used for IP at 6 µg per reaction. PSMD1 was also immunoprecipitated by a previous lot of this antibody (NBP3-30572 lot 1) and a second.inst a different epitope of PSMD1 . For blotting immunoprecipitated PSMD1, NBP3-30572 was used at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Rpn2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Rpn2 Antibody - BSA Free (NBP3-30572) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: between 903 and 953
• Assay Type: [Assay Type]
• Sample Volume: [Sample Volume]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PSMD1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-citrate/phosphate buffer, pH 7 to 8
• Preservative: 0.09% Sodium Azide
• Purity: Affinity purified

Alternate Names for Rpn2 Antibody - BSA Free

• 26S proteasome non-ATPase regulatory subunit 1
• 26S proteasome regulatory subunit RPN2
• 26S proteasome regulatory subunit S1
• 26S proteasome subunit p112
• MGC133040
• MGC133041
• P112
• proteasome (prosome, macropain) 26S subunit, non-ATPase, 1
• Rpn2
• S1

Background

Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signalling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Others are degraded via the vacuolar pathway which includes endosomes, lysosomes, and the endoplasmic reticulum. The 26S proteasome is an ATP-dependent, multisubunit (~31), barrel-shaped molecular machine with an apparent molecular weight of ~2.5 MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. An enzymatic cascade is responsible for the attachment of multiple ubiquitin molecules to lysine residues of proteins targeted for degradation. Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway. Some examples of affected proteins include those linked to cystic fibrosis, Angelmas's syndrome, and Liddle syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PSMD1