![Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with RPL32 antibody [HL2331] (NBP3-48676) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48676_rabbit-rpl32-mab-hl2331-western-blot-141120251834497.jpg "Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with RPL32 antibody [HL2331] (NBP3-48676) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
| Reactivity | Hu, Mu, Rt, Ca, ZeSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | HL2331 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | Novus Biologicals Rabbit RPL32 Antibody (HL2331) (NBP3-48676) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Full length of human RPL32 recombinant protein. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | RPL32 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | RPL32 |
![RPL32 antibody [HL2331] detects RPL32 protein on whole mount zebrafish by immunohistochemical analysis.Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.Green: RPL32 stained by RPL32 antibody [HL2331] (NBP3-48676) diluted at 1:100.Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70C](http://images.novusbio.com/fullsize/nbp3-48676_rabbit-rpl32-mab-hl2331-immunohistochemistry-141120251825200.jpg "RPL32 antibody [HL2331] detects RPL32 protein on whole mount zebrafish by immunohistochemical analysis.Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.Green: RPL32 stained by RPL32 antibody [HL2331] (NBP3-48676) diluted at 1:100.Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70C")
![RPL32 antibody [HL2331] detects RPL32 protein on whole mount zebrafish by immunohistochemical analysis.Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.Green: RPL32 stained by RPL32 antibody [HL2331] (NBP3-48676) diluted at 1:100.Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70C](http://images.novusbio.com/fullsize/nbp3-48676_rabbit-rpl32-mab-hl2331-immunohistochemistry-1411202518392023.jpg "RPL32 antibody [HL2331] detects RPL32 protein on whole mount zebrafish by immunohistochemical analysis.Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.Green: RPL32 stained by RPL32 antibody [HL2331] (NBP3-48676) diluted at 1:100.Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70C")
![RPL32 antibody [HL2331] detects RPL32 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.RPL32 stained by RPL32 antibody [HL2331] (NBP3-48676) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48676_rabbit-rpl32-mab-hl2331-immunohistochemistry-paraffin-141120251832292.jpg "RPL32 antibody [HL2331] detects RPL32 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.RPL32 stained by RPL32 antibody [HL2331] (NBP3-48676) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![RPL32 antibody [HL2331] detects RPL32 protein by immunohistochemical analysis.Sample: Paraffin-embedded dog tissues.RPL32 stained by RPL32 antibody [HL2331] (NBP3-48676) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48676_rabbit-rpl32-mab-hl2331-immunohistochemistry-paraffin-1411202518405416.jpg "RPL32 antibody [HL2331] detects RPL32 protein by immunohistochemical analysis.Sample: Paraffin-embedded dog tissues.RPL32 stained by RPL32 antibody [HL2331] (NBP3-48676) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with RPL32 antibody [HL2331] (NBP3-48676) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48676_rabbit-rpl32-mab-hl2331-western-blot-1411202518443412.jpg "Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with RPL32 antibody [HL2331] (NBP3-48676) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Whole Japanese medaka extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with RPL32 antibody [HL2331] (NBP3-48676) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48676_rabbit-rpl32-mab-hl2331-western-blot-1411202518243318.jpg "Whole Japanese medaka extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with RPL32 antibody [HL2331] (NBP3-48676) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![RPL32 antibody [HL2331] detects RPL32 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.RPL32 stained by RPL32 antibody [HL2331] (NBP3-48676) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48676_rabbit-rpl32-mab-hl2331-immunohistochemistry-paraffin-14112025184330.jpg "RPL32 antibody [HL2331] detects RPL32 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.RPL32 stained by RPL32 antibody [HL2331] (NBP3-48676) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Whole zebrafish extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with RPL32 antibody [HL2331] (NBP3-48676) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48676_rabbit-rpl32-mab-hl2331-western-blot-1411202518412920.jpg "Whole zebrafish extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with RPL32 antibody [HL2331] (NBP3-48676) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![RPL32 antibody [HL2331] detects RPL32 protein by immunofluorescent analysis.Sample: MDCK cells were fixed in ice-cold MeOH for 5 min.Green: RPL32 stained by RPL32 antibody [HL2331] (NBP3-48676) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-48676_rabbit-rpl32-mab-hl2331-immunocytochemistry-immunofluorescence-1411202518294116.jpg "RPL32 antibody [HL2331] detects RPL32 protein by immunofluorescent analysis.Sample: MDCK cells were fixed in ice-cold MeOH for 5 min.Green: RPL32 stained by RPL32 antibody [HL2331] (NBP3-48676) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .")
![RPL32 antibody [HL2331] detects RPL32 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: RPL32 stained by RPL32 antibody [HL2331] (NBP3-48676) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-48676_rabbit-rpl32-mab-hl2331-immunocytochemistry-immunofluorescence-1411202518412911.jpg "RPL32 antibody [HL2331] detects RPL32 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: RPL32 stained by RPL32 antibody [HL2331] (NBP3-48676) diluted at 1:500.Blue: Fluoroshield with DAPI .")
![Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with RPL32 antibody [HL2331] (NBP3-48676) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48676_rabbit-rpl32-mab-hl2331-western-blot-141120251832417.jpg "Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with RPL32 antibody [HL2331] (NBP3-48676) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot Galectin-3 Antibody [Unconjugated]](https://images.novusbio.com/images2/Galectin3_AF1197_Western_Blot_21471.jpg)
![Immunohistochemistry Galectin-3 Antibody [Unconjugated]](https://images.novusbio.com/images2/Galectin-3_AF1197_Immunohistochemistry_7115.jpg)
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")