RPIP8 Antibody (OTI3C6) - Azide and BSA Free

Western Blot: RPIP8 Antibody (OTI3C6) - Azide and BSA Free [NBP2-73943] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPIP8.

Immunohistochemistry: RPIP8 Antibody (OTI3C6) - Azide and BSA Free [NBP2-73943] - Analysis of Adenocarcinoma of Human colon tissue.

Immunohistochemistry: RPIP8 Antibody (OTI3C6) - Azide and BSA Free [NBP2-73943] - Analysis of Human pancreas tissue.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: OTI3C6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

RPIP8 Antibody (OTI3C6) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human RUNDC3A(NP_006686) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: RUNDC3A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:1000
• Theoretical MW: 45.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for RPIP8 Antibody (OTI3C6) - Azide and BSA Free

• Rap2-interacting protein 8
• RAP2IPRUN domain-containing protein 3A
• RPIP-8
• RPIP8RaP2 interacting protein 8
• RUN domain containing 3A

Background

RPIP8 is a gene that codes for a protein that has four isoforms, with lengths of 446, 400, 405, and 364 amino acids long and weights of approximately 50, 45, 46, and 41 kDa, respectively. RPIP8 functions as an effector of RAP2A in neuronal cells. Current research is being done on diseases and disorders relating to this gene including dementia, ataxia, malaria, and neuronitis. RPIP8 has also been shown to have interactions with RAP2B, GFI1B, EIF3E, AP2M1, and AP1M1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RUNDC3A