PARG1 Antibody

Images

 
Independent Antibodies: Western Blot: PARG1 Antibody [NBP1-05989] - Whole cell lysate from HeLa, 293T, and mouse NIH3T3 cells. PARG1 was also immunoprecipitated by rabbit anti-PARG1 antibody NBP1-05988.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

PARG1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1211 and 1261 of human PTPL1-associated RhoGAP 1 (NP_004806.3).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ARHGAP29
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
Application Notes
ICC/IF, IHC reactivity reported in scientific literature (PMID: 23008150).
Control
PARG1 Overexpression Lysate
Publications
Read Publications using
NBP1-05989 in the following applications:

  • 3 publications
  • IHC
    2 publications
  • WB
    5 publications

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PARG1 Antibody

  • PARG1PTPL1-associated RhoGAP 1 (PARG1)
  • PTPL1-associated RhoGAP protein 1
  • Rho GTPase activating protein 29
  • rho GTPase-activating protein 29
  • Rho-type GTPase-activating protein 29
  • RP11-255E17.1

Background

PARG1 (PTPL1-associated RhoGAP protein 1) is a GTPase-activating protein (GAP) for Rho and Rap2. PARG1 has recently been identified as a candidate tumor suppressor in mantle-cell lymphoma. Alternative names for PARG1 include Rho GTPase-activating protein 29 and ARHGAP29.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PARG1 Antibody (NBP1-05989)(8)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ICC/IF, IHC, WB.


Filter By Application
ICC/IF
(3)
IHC
(2)
WB
(5)
All Applications
Filter By Species
Human
(3)
Mouse
(4)
All Species
Showing Publications 1 - 8 of 8.
Publications using NBP1-05989 Applications Species
Kolb K, Hellinger J, Kansy M et al. Influence of ARHGAP29 on the Invasion of Mesenchymal-Transformed Breast Cancer Cells Cells Dec 5 2020 [PMID: 33291460] (WB, Human) WB Human
Leinhos L Myofibroblast differentiation in hypoxia: a novel role for ArhGAP29 Thesis (WB) WB
Xu Q, Duan H, Gan L et al. MicroRNA-1291 promotes endometrial fibrosis by regulating the ArhGAP29-RhoA/ROCK1 signaling pathway in a murine model Mol Med Rep 2017 Aug 10 [PMID: 28849001] (IHC, Mouse) IHC Mouse
Paul BJ, Palmer K, Sharp JC et al. ARHGAP29 Mutation Is Associated with Abnormal Oral Epithelial Adhesions J. Dent. Res. 2017 Aug 01 [PMID: 28817352] (WB, Mouse) WB Mouse
Post A, Pannekoek WJ, Ponsioen B et al. Rap1 spatially controls ArhGAP29 to inhibit Rho signaling during endothelial barrier regulation. Mol. Cell. Biol. 2015 May 11 [PMID: 25963656] (ICC/IF, Human) ICC/IF Human
Biggs LC, Naridze RL, Demali KA et al. Interferon regulatory factor 6 regulates keratinocyte migration. J. Cell. Sci. 2014 Apr 28 [PMID: 24777480] (WB, ICC/IF, Mouse) WB, ICC/IF Mouse
Post A, Pannekoek WJ, Ross SH et al. Rasip1 mediates Rap1 regulation of Rho in endothelial barrier function through ArhGAP29. Proc Natl Acad Sci U S A 2013 Jul 9 [PMID: 23798437] (WB, Human) WB Human
Leslie EJ, Mansilla MA, Biggs LC et al. Expression and mutation analyses implicate ARHGAP29 as the etiologic gene for the cleft lip with or without cleft palate locus identified by genome-wide association on chromosome 1p22 Birth Defects Res A Clin Mol Teratol 2012 Nov [PMID: 23008150] (IHC, ICC/IF, Mouse) IHC, ICC/IF Mouse

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PARG1 Antibody (NBP1-05989) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ARHGAP29
Entrez
Uniprot