PARG1 Antibody - BSA Free

Western Blot: PARG1 Antibody [NBP1-05989] - Whole cell lysate from HeLa, 293T, and mouse NIH3T3 cells. PARG1 was also immunoprecipitated by rabbit anti-PARG1 antibody NBP1-05988.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

PARG1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit PARG1 Antibody - BSA Free (NBP1-05989) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. Anti-PARG1 Antibody: Cited in 11 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1211 and 1261 of human PTPL1-associated RhoGAP 1 (NP_004806.3).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ARHGAP29
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: ICC/IF, IHC reactivity reported in scientific literature (PMID: 23008150). Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.
• Control:

  PARG1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PARG1 Antibody - BSA Free

• PARG1PTPL1-associated RhoGAP 1 (PARG1)
• PTPL1-associated RhoGAP protein 1
• Rho GTPase activating protein 29
• rho GTPase-activating protein 29
• Rho-type GTPase-activating protein 29
• RP11-255E17.1

Background

PARG1 (PTPL1-associated RhoGAP protein 1) is a GTPase-activating protein (GAP) for Rho and Rap2. PARG1 has recently been identified as a candidate tumor suppressor in mantle-cell lymphoma. Alternative names for PARG1 include Rho GTPase-activating protein 29 and ARHGAP29.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PARG1 Antibody (NBP1-05989)(12)

Publications using NBP1-05989

• Rhea L, Reeb T, Adelizzi E et Al. ARHGAP29 promotes keratinocyte proliferation and migration in vitro and is dispensable for in vivo wound healing Dev Dyn 2024-11-19 [PMID: 39560169]
• MAIKE KANSY, KATHARINA WERT, KATHARINA KOLB, JULIA GALLWAS, CARSTEN GRÜNDKER ARHGAP29 Is Involved in Increased Invasiveness of Tamoxifen-resistant Breast Cancer Cells and its Expression Levels Correlate With Clinical Tumor Parameters of Breast Cancer Patients Cancer Genomics & Proteomics 2024-07-03 [PMID: 38944420]
• Reeb T, Rhea L, Adelizzi E et al. ARHGAP29 is required for keratinocyte proliferation and migration bioRxiv : the preprint server for biology 2023-02-01 [PMID: 36778214] (WB, Human)
• Li MJ, Shi JY, Zhang BH et al. Targeted re-sequencing on 1p22 among non-syndromic orofacial clefts from Han Chinese population Frontiers in genetics 2022-08-17 [PMID: 36061182] (IHC-P, WB, Human)
• Xu Q, Duan H, Gan L et al. MicroRNA-1291 promotes endometrial fibrosis by regulating the ArhGAP29-RhoA/ROCK1 signaling pathway in a murine model Mol Med Rep 2017-08-10 [PMID: 28849001] (IF/IHC, Mouse)
• Paul BJ, Palmer K, Sharp JC et al. ARHGAP29 Mutation Is Associated with Abnormal Oral Epithelial Adhesions J. Dent. Res. 2017-08-01 [PMID: 28817352] (WB, Mouse)
• Post A, Pannekoek WJ, Ponsioen B et al. Rap1 spatially controls ArhGAP29 to inhibit Rho signaling during endothelial barrier regulation. Mol. Cell. Biol. 2015-05-11 [PMID: 25963656] (ICC/IF, Human)
• Biggs LC, Naridze RL, Demali KA et al. Interferon regulatory factor 6 regulates keratinocyte migration. J. Cell. Sci. 2014-04-28 [PMID: 24777480] (WB, ICC/IF, Mouse)
• Post A, Pannekoek WJ, Ross SH et al. Rasip1 mediates Rap1 regulation of Rho in endothelial barrier function through ArhGAP29. Proc Natl Acad Sci U S A 2013-07-09 [PMID: 23798437] (WB, Human)
• Leslie EJ, Mansilla MA, Biggs LC et al. Expression and mutation analyses implicate ARHGAP29 as the etiologic gene for the cleft lip with or without cleft palate locus identified by genome-wide association on chromosome 1p22 Birth Defects Res A Clin Mol Teratol 2012-11-01 [PMID: 23008150] (IF/IHC, ICC/IF, Mouse)
• Leinhos L Myofibroblast differentiation in hypoxia: a novel role for ArhGAP29 Thesis (WB)
• Kolb K, Hellinger J, Kansy M et al. Influence of ARHGAP29 on the Invasion of Mesenchymal-Transformed Breast Cancer Cells Cells 2020-12-05 [PMID: 33291460] (WB, Human)

Bioinformatics

• Gene Symbol: ARHGAP29
• Entrez:
  • 9411(Human)
  • 214137(Mouse)
• Uniprot:
  • Q52LW3(Human)