GPR64 Antibody [Unconjugated]


Western blot shows lysates of rat epididymis tissue and mouse epididymis tissue. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human/Mouse/Rat GPR64 Antigen Affinity-purified Polyclonal Antibody (Catalog # more
HEK293 human embryonic kidney cell line transfected with (A) Human GPR64 or (B) Human GPR75 and eGFP was stained with Sheep Anti-Human GPR64 Affinity-Purified Polyclonal Antibody (Catalog # AF7977) followed by more
GPR64 was detected in immersion fixed paraffin-embedded sections of human epididymus using Sheep Anti-Human/Mouse/Rat GPR64 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7977) at 3 µg/mL overnight at 4 more

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, IHC

Order Details

View Available Formulations
Catalog# & Formulation Size Price

GPR64 Antibody [Unconjugated] Summary

Chinese hamster ovary cell line CHO-derived recombinant human GPR64
Leu38-Asn64, Glu68-Thr553
Accession # NP_001073328
Detects human, mouse, and rat GPR64 in direct ELISA and Western blots.
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 2 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GPR64 Antibody [Unconjugated]

  • EDDM6
  • epididymis-specific (seven transmembrane family)
  • G protein-coupled receptor 64
  • GPR64
  • HE6
  • MGC104454
  • MGC138738
  • TM7LN2


GPR64 (G-Protein coupled Receptor 64; also HE6) is a 110 kDa (predicted) member of the B class of GCPRs. Within this class GPR64 belongs to a Large N-termini family-B 7-transmembrane (LNB-7TM) subclass of receptors (also known as adhesion-GCPRs). GPR64 has restricted expression, being found in stereocilia cell membranes of epididymal caput epithelial cells and, to a limited extent, on osteoblasts. The function of GPR64 is somewhat unclear, but in the epididymis, it may be involved in fluid transport. Mature human GPR64 is a 980 amino acid (aa) 7-TM glycoprotein (SwissProt Q8IZP9). It contains an extended extracellular N-teminus (aa 38-627), seven consecutive TM segments (aa 628-878) and a C-terminal cytoplasmic tail (aa 879-1010). The extended extracellular region possesses a juxtamembrane GPS domain (aa 567-618) that serves as a proteolytic cleavage site. Enzymatic activity here generates a 180 kDa soluble form that stays associated with a 40 kDa membrane-embedded fragment. Notably, isolation of the membrane fragment gives rise to oligomers that run at > 250 kDa in SDS-PAGE. There are multiple splice variants. The one used for immunization to generate this antibody contains a deletion of aa 88-101 (RefSeq NP_001073328). Four other splice forms show single block deletions of aa 65-67, 51-66, 52-75, and 906-956, respectively. Three others possesses aa substitutions; a 20 aa block for aa 52-101, and a common 12 aa block that can substitute for either aa 68-101 or aa 52-101. Over aa 38-64 and 68-553 of RefSeq NP_001073328, human and mouse GPR64 share 69% aa sequence identity.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: IHC, IHC-P
Species: Hu, Pm
Applications: IHC, IHC-P, ICC
Species: Hu
Applications: WB, Simple Western, Flow, CyTOF-ready
Species: Hu, Mu
Applications: IHC, IHC-Fr, IHC-P
Species: Hu, Pm, Pm
Applications: IHC, IHC-P
Species: Hu, Mu
Applications: WB, ICC/IF, IHC-P
Species: Hu, Mu
Applications: WB, Flow, CyTOF-ready, ICC
Species: Hu, Rt, Pm
Applications: IHC, IHC-P
Species: Mu
Applications: WB, IHC, ELISA(Cap), ELISA(Det), ICC, Neut, ELISA(Sta)
Species: Hu, Rt, Po, Bv
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Pm-Cm, RM
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vivo, CyTOF-ready, Dual ISH-IHC
Species: Hu
Applications: WB, IHC, IHC-P, PEP-ELISA
Species: Hu
Applications: IHC, IHC-P

Publications for GPR64 Antibody (AF7977) (0)

There are no publications for GPR64 Antibody (AF7977).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for GPR64 Antibody (AF7977) (0)

There are no reviews for GPR64 Antibody (AF7977). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for GPR64 Antibody (AF7977) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies


Isotype Controls

Other Available Formats

Additional GPR64 Products

Bioinformatics Tool for GPR64 Antibody (AF7977)

Discover related pathways, diseases and genes to GPR64 Antibody (AF7977). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for GPR64 Antibody (AF7977)

Discover more about diseases related to GPR64 Antibody (AF7977).

Pathways for GPR64 Antibody (AF7977)

View related products by pathway.

PTMs for GPR64 Antibody (AF7977)

Learn more about PTMs related to GPR64 Antibody (AF7977).

Blogs on GPR64

There are no specific blogs for GPR64, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our GPR64 Antibody [Unconjugated] and receive a gift card or discount.


Gene Symbol GPR64