RPA14 Antibody (14.2) - Azide and BSA Free

Western Blot: RPA14 Antibody (14.1) [NB100-160] - Sample (30 ug of whole cell lysate) A: Molt-4 15% SDS PAGE, antibody diluted at 1:500.

• Reactivity: Hu, Mu
• Applications: WB, IP
• Clone: 14.2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

RPA14 Antibody (14.2) - Azide and BSA Free Summary

• Immunogen: Full-length human RPA-14 expressed in E. coli..
• Localization: Nuclear
• Specificity: NB100-160 is specific for human and mouse RPA14.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: RPA3
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 0.1-2 ug/ml
  • Western Blot 0.1 - 2 ug/ml
• Theoretical MW: 14 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for RPA14 Antibody (14.2) - Azide and BSA Free

• REPA3Replication factor A protein 3
• replication protein A 14 kDa subunit
• replication protein A3 (14kD)
• replication protein A3, 14kDa
• RF-A protein 3
• RP-A p14
• RPA14

Background

Replication protein A (RPA) is a single-stranded DNA binding protein. Human RPA is a heterotrimeric protein containing subunits of 70, 32 and 14 kDa. This protein complex is highly conserved in eukaryotes and is essential in DNA replication, homologous recombination and nucleotide excision repair. RPA has been shown to be a target for DNA-dependent protein kinase (DNA-PK) ataxia telangiectasia-mutated gene (ATM) and a cyclin dependent protein kinase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RPA14 Antibody (NB100-160)(2)

Publications using NB100-160

• Meetei AR, Sechi S, Wallisch M et al. A multiprotein nuclear complex connects Fanconi anemia and Bloom syndrome. Mol Cell Biol 2003-05-01 [PMID: 12724401]
• Sukhodolets KE, Hickman AB, Agarwal SK et al. The 32-kilodalton subunit of replication protein A interacts with menin, the product of the MEN1 tumor suppressor gene. Mol Cell Biol 2003-01-01 [PMID: 12509449] (WB)

Bioinformatics

• Gene Symbol: RPA3
• Entrez:
  • 6119(Human)
  • 68240(Mouse)