RP1 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: RP1 Antibody [NBP2-22149] - RP1 antibody was tested in ARPE-19 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Immunohistochemistry: RP1 Antibody [NBP2-22149] - IHC analysis of RP1 in mouse retina.

• Reactivity: Hu, Mu, Pm
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 2.0 mg/ml

RP1 Antibody - BSA Free Summary

• Immunogen: A synthetic peptide made to a C-terminal portion of the human RP1 protein (between residues 2000-2156) [UniProt P56715]
• Localization: Cytoplasm.
• Predicted Species: Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:100
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Paraffin 1:400
  • Western Blot 2.0 ug/ml
• Application Notes: This RP1 antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry on paraffin embedded sections. In Western blot a band was observed ~ 240 kDa. In ICC/IF cytoplasmic staining was observed in Arpe-19 cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 240 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Predicted to react with monkey based upon 100% sequence homology.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 2.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RP1 Antibody - BSA Free

• DCDC4A
• FLJ50293
• FLJ55454
• oxygen-regulated protein 1
• retinitis pigmentosa 1 (autosomal dominant)
• retinitis pigmentosa 1 protein
• retinitis pigmentosa RP1 protein

Background

Retinitis pigmentosa 1 (RP1), also known as oxygen-regulated protein 1, retinitis pigmentosa 1 protein, ORP1, and DCDC4A, regulates the stability and length of microtubule based axoneme of photoreceptors. RP1 is critical for the differentiation of photoreceptor cells, and RP1 also influences the photosensitivity and outer segment morphogenesis of rod photoreceptors. Similarly, RP1 controls the correct orientation and higher order stacking of the outer segment disks along the photoreceptor axoneme. RP1 is found in the cytoplasm and has only been reported in the retina. RP1 is subject to phosphorylation, and mutations in RP1 cause autosomal recessive and dominant retinitis pigmentosa. Both of these diseases lead to degeneration of the retinal photoreceptor cells resulting in night blindness, loss of peripheral visual field, and eventually loss of central vision (PMID: 10391211).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RP1
• Entrez:
  • 6101(Human)
  • 19888(Mouse)
• Uniprot:
  • P56715(Human)
  • P56716(Mouse)