ROCK2 Antibody (1E12) - Azide and BSA Free

Western Blot: ROCK2 Antibody (1E12) [H00009475-M02] - ROCK2 monoclonal antibody (M02), clone 1E12 Analysis of ROCK2 expression in Hela S3 NE.

Immunocytochemistry/Immunofluorescence: ROCK2 Antibody (1E12) [H00009475-M02] - Analysis of monoclonal antibody to ROCK2 on HeLa cell. Antibody concentration 30 ug/ml.

Immunohistochemistry-Paraffin: ROCK2 Antibody (1E12) [H00009475-M02] - Analysis of monoclonal antibody to ROCK2 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 1.5 ug/ml.

Sandwich ELISA: ROCK2 Antibody (1E12) [H00009475-M02] - Detection limit for recombinant GST tagged ROCK2 is 0.3 ng/ml as a capture antibody.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 1E12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

ROCK2 Antibody (1E12) - Azide and BSA Free Summary

• Immunogen: ROCK2 (NP_004841, 1279 a.a. ~ 1388 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KPLWHMFKPPPALECRRCHIKCHKDHMDKKEEIIAPCKVYYDISTAKNLLLLANSTEEQQKWVSRLVKKIPKKPPAPDPFARSSPRTSMKIQQNQSIRRPSRQLAPNKPS
• Specificity: ROCK2 (1E12)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ROCK2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, ELISA and immunohistochemistry (paraffin).

Reactivity Notes

Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ROCK2 Antibody (1E12) - Azide and BSA Free

• coiled-coil-containing protein kinase 2
• EC 2.7.11
• EC 2.7.11.1
• KIAA0619
• p164 ROCK-2
• Rho kinase 2
• rho-associated protein kinase 2
• Rho-associated, coiled-coil containing protein kinase 2
• ROCK2
• ROCK-II
• ROKalpha

Background

The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ROCK2 Antibody (H00009475-M02)(4)

Publications using H00009475-M02

• Chen X, Fansler MM, Janjoš U, Ule J et Al. The FXR1 network acts as a signaling scaffold for actomyosin remodeling Cell 2024-08-06 [PMID: 39106863]
• Elena P, Francesca M, Lorenzo M et al. Identification of Homoharringtonine as a potent inhibitor of glioblastoma cell proliferation and migration. Transl Res. 2022-07-03 [PMID: 35788055]
• Chen SC, Huang SY, Lin WT et al. Aortic smooth muscle cell alterations in mice systemically exposed to arsenic. Heart Vessels 2015-07-02 [PMID: 26135927]
• Chen SC, Liu CC, Huang SY, Chiou SJ. Vascular hyperpermeability in response to inflammatory mustard oil is mediated by Rho kinase in mice systemically exposed to arsenic. Microvasc Res. 2011-06-16 [PMID: 21703283]

Bioinformatics

• Gene Symbol: ROCK2
• Entrez:
  • 9475(Human)
• OMIM:
  • 604002(Human)
• Uniprot:
  • NP_004841(Human)