RNGTT Antibody

Images

 
Western Blot: RNGTT Antibody [NBP1-49973] - Detection of Human and Mouse RNGTT by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP ...read more
Immunohistochemistry: RNGTT Antibody [NBP1-49973] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- RNGTT used at a dilution of 1:200 (1ug/ml). Detection: Vector Laboratories ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
 

Independent Antibodies

 

Biological Strategies

     

Order Details

RNGTT Antibody Summary

Immunogen
The immunogen for this product maps to a region between residue 350 and 400 of human RNA Guanylyltransferase and 5'-Phosphatase using the numbering given in entry NP_003791.3 (GeneID 8732).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RNGTT
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Immunoprecipitation 2-5 ug/mg
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-49973 in the following applications:

Publications
Read Publication using
NBP1-49973 in the following applications:

  • IP
    1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RNGTT Antibody

  • CAP1AmRNA-capping enzyme
  • hCAP
  • HCE1DKFZp686J2031
  • HCEHCAP1
  • RNA guanylyltransferase and 5'-phosphatase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RNGTT Antibody (NBP1-49973)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IP, WB.


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IP
(1)
WB
(1)
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Filter By Species
Human
(1)
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Review for RNGTT Antibody (NBP1-49973) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: IP.

Reviews using NBP1-49973:
Filter by Applications
IP
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Immunoprecipitation RNGTT NBP1-49973
Enlarge
reviewed by:
Anonymous
IP Human 07/22/2014
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Summary

ApplicationImmunoprecipitation
Sample TestedCytoplasmic extract from 293 cell
SpeciesHuman
LotA1

Blocking

Blocking DetailsN/A

Primary Anitbody

Dilution RatioN/A

Details

Detection NotesAfter IP, I have done guanylation assay to detect the protein since western blot is not sufficient to detect the low expression
Immunoprecipitation DetailsDynabeads Protein G (Life Technologies), 30 mg/ml, 16 h, 4.C, washing with IPP150 (20 mM Tris-Cl, pH7.5, 150 mM NaCl, 0.1% NP40
Elution Description2X SDS-PAGE loading buffer, 95.C 5 mins

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for RNGTT Antibody (NBP1-49973) (0)

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Secondary Antibodies

 

Isotype Controls

Additional RNGTT Products

Bioinformatics Tool for RNGTT Antibody (NBP1-49973)

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Diseases for RNGTT Antibody (NBP1-49973)

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Recent Reviews

5
5
1
4
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2
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1
0

Anonymous
07/22/2014
Application: IP
Species: Human

Bioinformatics

Gene Symbol RNGTT