RNGTT Antibody

Western Blot: RNGTT Antibody [NBP1-49972] - Detection of Human and Mouse RNGTT by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-RNGTT antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. RNGTT was also immunoprecipitated by rabbit anti-RNGTT antibody which recognizes a downstream epitope. For blotting immunoprecipitated RNGTT was used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Immunohistochemistry-Paraffin: RNGTT Antibody [NBP1-49972] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- RNGTT used at a dilution of 1:200 (1ug/ml). Detection: Vector Laboratories ImmPACT NovaRED Peroxidase Substrate

Product Discontinued

RNGTT Antibody Summary

• Immunogen: The immunogen for this product maps to a region between residue 1 and 50 of human RNA Guanylyltransferase and 5'-Phosphatase using the numbering given in entry NP_003791.3 (GeneID 8732).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RNGTT
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Paraffin 1:100 - 1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RNGTT Antibody

• CAP1AmRNA-capping enzyme
• hCAP
• HCE1DKFZp686J2031
• HCEHCAP1
• RNA guanylyltransferase and 5'-phosphatase

Background

Bifunctional mRNA-capping enzyme exhibiting RNA 5'-triphosphatase activity in the N-terminal part and mRNA guanylyltransferase activity in the C-terminal part. Catalyzes the first two steps of cap formation: by removing the gamma-phosphate from the 5'-triphosphate end of nascent mRNA to yield a diphosphate end, and by transferring the gmp moiety of GTP to the 5'-diphosphate terminus

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RNGTT Antibody (NBP1-49972)(2)

Publications using NBP1-49972

• Trotman JB, Agana BA, Giltmier AJ et al. RNA-binding proteins and heat-shock protein 90 are constituents of the cytoplasmic capping enzyme interactome. J. Biol. Chem. 2018-08-30 [PMID: 30166341] (WB, Human)
• Housden BE, Valvezan AJ, Kelley C et al. Identification of potential drug targets for tuberous sclerosis complex by synthetic screens combining CRISPR-based knockouts with RNAi. Sci Signal 2015-09-08 [PMID: 26350902] (WB)

Bioinformatics

• Gene Symbol: RNGTT