RNF7 Antibody

Product Discontinued

RNF7 Antibody Summary

• Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 102-113 of Human RNF7 (C-terminal) coupled to KLH.
• Specificity: This product reacts with human, mouse, C.elgans and zebra fish ROC2. Cross reactivity may also occur with ROC2 from other sources.
• Isotype: Serum
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RNF7
• Purity: Sterile filtered

Applications/Dilutions

• Dilutions:
  • ELISA 1:2000-1:10000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation
  • Western Blot 1:500-1:1000
• Application Notes: The antibody immunoprecipitates in vitro translated protein and protein from overexpressing cell lysates . Coimmunoprecipitation of related proteins does occur. A 12.6 kDa band corresponding - human ROC2 is detected. Most cell lines expressing ROC2 can be used as a positive control. Researchers should determine optimal titers for other applications.

Reactivity Notes

Cross reactivity with RNF7 from other sources may occur but has not been tested.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Antiserum
• Preservative: 0.01% Sodium Azide
• Purity: Sterile filtered

Alternate Names for RNF7 Antibody

• CKBBP1zinc RING finger protein SAG
• CKII beta-binding protein 1
• RBX2
• Regulator of cullins 2
• ring finger protein 7rbx2
• ROC2RING-box protein 2
• SAGsensitive to apoptosis, zinc RING finger protein SAG, regulator of cullins 2
• Sensitive to apoptosis gene protein

Background

The protein encoded by this gene is a highly conserved ring finger protein. It is an essential subunit of SKP1-cullin/CDC53-F box protein ubiquitin ligases, which are a part of the protein degradation machinery important for cell cycle progression and signal transduction. This protein interacts with, and is a substrate of, casein kinase II (CSNK2A1/CKII). The phosphorylation of this protein by CSNK2A1 has been shown to promote the degradation of IkappaBalpha (CHUK/IKK-alpha/IKBKA) and p27Kip1(CDKN1B). Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RNF7 Antibody (NBP1-78049)(3)

Publications using NBP1-78049

• Wang X, Liu X, Xue L et al. Hunting for serine 276-phosphorylated p65. J Biomed Biotechnol 2010-01-01 [PMID: 20204068]
• Sheldon M, Rice DS, D'Arcangelo G et al. Toll-like receptor 2 mediates apolipoprotein CIII-induced monocyte activation. Circ Res 2008-12-01 [PMID: 18974386]
• Manna SK, Aggarwal RS, Sethi G et al. CXCL5 promotes prostate cancer progression. Neoplasia 2008-03-01 [PMID: 18320069]

Bioinformatics

• Gene Symbol: RNF7
• Uniprot:
  • Q9UBF6(Human)