RNF139 Antibody - BSA Free

Immunohistochemistry-Paraffin: RNF139 Antibody [NBP1-83212] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.

Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.

Staining of human kidney shows strong cytoplasmic positivity in cells in glomeruli.

Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

RNF139 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: PEIKGSRLQEINDVCAICYHEFTTSARITPCNHYFHALCLRKWLYIQDTCPMCHQKVYIEDDIKDNSNVSNNNGFIPPNETPEEAVREAAAESDRELNEDDSTDCDDDVQRERNGVIQHTGAAAEEF
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RNF139
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  RNF139 Protein (NBP1-83212PEP)

Reactivity Notes

Mouse (88%), Rat (88%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for RNF139 Antibody - BSA Free

• EC 6.3.2.-
• HRCA1
• patched related protein translocated in renal cancer
• RCA1multiple membrane spanning receptor TRC8
• ring finger protein 139MGC31961
• Translocation in renal carcinoma on chromosome 8 protein
• TRC8E3 ubiquitin-protein ligase RNF139

Background

RNF139 is encoded by this gene is a multi-membrane spanning protein containing a RING-H2 finger. This protein is located in the endoplasmic reticulum, and has been shown to possess ubiquitin ligase activity. This gene was found to be interrupted by a t(3:8) translocation in a family with hereditary renal and non-medulary thyroid cancer. Studies of the Drosophila counterpart suggested that this protein may interact with tumor suppressor protein VHL, as well as with COPS5/JAB1, a protein responsible for the degradation of tumor suppressor CDKN1B/P27KIP.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RNF139