RNASE3 Antibody (5G4)

Images

 
Western Blot: RNASE3 Antibody (5G4) [NBP2-76905] - Western blot analysis of Ribonuclease 3 on U937 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. ...read more
Immunocytochemistry/ Immunofluorescence: RNASE3 Antibody (5G4) [NBP2-76905] - ICC staining Ribonuclease 3 in SHG-44 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at ...read more
Immunohistochemistry: RNASE3 Antibody (5G4) [NBP2-76905] - Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Ribonuclease 3 antibody. The section was pre-treated using heat mediated ...read more
Flow Cytometry: RNASE3 Antibody (5G4) [NBP2-76905] - Flow cytometric analysis of Ribonuclease 3 was done on Hela cells. The cells were fixed, permeabilized and stained with Ribonuclease 3 antibody at 1/100 dilution ...read more
Immunocytochemistry/ Immunofluorescence: RNASE3 Antibody (5G4) [NBP2-76905] - ICC staining Ribonuclease 3 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at ...read more
Immunohistochemistry: RNASE3 Antibody (5G4) [NBP2-76905] - Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Ribonuclease 3 antibody. The section was pre-treated using heat mediated ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
5G4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
2 mg/ml

Order Details

RNASE3 Antibody (5G4) Summary

Immunogen
Recombinant protein within human Ribonuclease 3 aa 28-120.
Localization
Secreted.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RNASE3
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
Theoretical MW
18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 1%BSA, 50% Glycerol
Preservative
0.05% Sodium Azide
Concentration
2 mg/ml
Purity
Protein G purified

Alternate Names for RNASE3 Antibody (5G4)

  • eosinophil cationic protein
  • ECP
  • ribonuclease, RNase A family, 3
  • RNS3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for RNASE3 Antibody (NBP2-76905) (0)

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol RNASE3