RNase L Antibody (HL2340) - Azide and BSA Free

Western Blot: RNase L Antibody (HL2340) - Azide and BSA Free [NBP3-25639] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RNase L antibody [HL2340] (NBP3-25639) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: RNase L Antibody (HL2340) - Azide and BSA Free [NBP3-25639] - RNase L antibody [HL2340] detects RNase L protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: RNase L stained by RNase L antibody [HL2340] (NBP3-25639) diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI .

RNase L antibody [HL2340] detects RNase L protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded rat kidney.RNase L stained by RNase L antibody [HL2340] (NBP3-25639) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

RNase L antibody [HL2340] detects RNase L protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded mouse kidney.RNase L stained by RNase L antibody [HL2340] (NBP3-25639) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: HL2340
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

RNase L Antibody (HL2340) - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit RNase L Antibody (HL2340) - Azide and BSA Free (NBP3-25639) is a recombinant monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragment of human RNase L
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: RNASEL
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500-1:3000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for RNase L Antibody (HL2340) - Azide and BSA Free

• 2-5A-dependent ribonuclease
• 2-5A-dependent RNase
• 2'-5'-oligoisoadenylate synthetase-dependent
• EC 3.1.26.-
• HPC1
• interferon-induced 2-5A-dependent RNase
• MGC104972
• MGC133329
• PRCA1
• prostate cancer 1
• Ribonuclease 4
• ribonuclease L (2'-5'-oligoisoadenylate synthetase-dependent)
• Ribonuclease L
• RNase L
• RNS4DKFZp781D08126

Background

RNase L is a virally induced endoribonuclease that is also the terminal factor of the anti-viral action of interferon. Activation of RNase L results in inhibition of viral proliferation through total RNA degradation. Activation of RNase L has also been determined to degrade RNA, thus, initiating a cellular stress response, inducing apoptosis. Since mutations in RNase L relate to an increased incidence of prostate cancer, the activation of RNase L is thought to function in counteracting prostate cancer, via apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RNASEL