RNA Polymerase II/POLR2A Antibody

Product Discontinued

RNA Polymerase II/POLR2A Antibody Summary

• Immunogen: The antiserum was produced against synthesized peptide derived from human POLR2A. The immunogen region is: C-terminal.
• Specificity: POLR2A (Ab-1619) Antibody detects endogenous levels of total POLR2A protein.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: POLR2A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500-1:1000

Reactivity Notes

Human,Mouse, Rat

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (without Mg2+ and Ca2+, pH 7.4), 0.15M NaCl and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RNA Polymerase II/POLR2A Antibody

• DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kdsubunit
• DNA-directed RNA polymerase II subunit A
• DNA-directed RNA polymerase II subunit RPB1
• DNA-directed RNA polymerase III largest subunit
• EC 2.7.7.48
• EC 2.7.7.6
• hRPB220
• hsRPB1
• MGC75453
• POLR2
• POLR2A
• POLRA
• polymerase (RNA) II (DNA directed) polypeptide A (220kD)
• polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
• RNA Pol 2
• RNA Polymerase 2
• RNA polymerase II subunit B1
• RNA Polymerase II
• RNA-directed RNA polymerase II subunit RPB1
• RNAPII
• RPB1
• RPBh1
• RpIILS
• RPO2
• RPOL2

Background

DNA-dependent RNA polymerase II (Pol II), a complex multisubunit enzyme, is responsible for the transcription of protein-coding genes. This complex interacts with the promoter regions of genes as well as with a variety of elements and transcription factors to determine essentially all of the parameters that govern transcription. A fraction of the large subunit of Pol II is ubiquitinated after exposing cells to UV radiation or cisplatin suggesting that ubiquitination of Pol II plays a role in the recognition and/or repair of damage to actively transcribed genes (1). Evidence demonstrates that pol II transcription termination involves two distinct and temporally separate events. The first event, termed pretermination cleavage (PTC), is mediated by sequence tracts located downstream of the poly(A) site which appear to promote heterogeneous cleavage of the nascent transcript. The second event, in which pol II disengages from the DNA template, requires that polymerase has transcribed both a PTC sequence tract and a functional poly(A) site (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: POLR2A
• Uniprot:
  • P24928(Human)