RIOK1 Antibody - BSA Free

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Immunohistochemistry-Paraffin: RIOK1 Antibody [NBP2-47301] - Analysis in human testis and pancreas tissues using NBP2-47301 antibody. Corresponding RIOK1 RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: RIOK1 Antibody [NBP2-47301] - Immunofluorescent staining of human cell line PC-3 shows localization to nucleoli, nuclear speckles & cytosol.
Immunohistochemistry-Paraffin: RIOK1 Antibody [NBP2-47301] - Staining of human testis shows moderate cytoplasmic positivity in subset of cells in seminiferous ducts.
Immunohistochemistry-Paraffin: RIOK1 Antibody [NBP2-47301] - Staining of human skeletal muscle shows low expression as expected.
Immunohistochemistry-Paraffin: RIOK1 Antibody [NBP2-47301] - Staining of human pancreas shows no cytoplasmic positivity in exocrine glandular cells as expected.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free

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Catalog# & Formulation Size Price

RIOK1 Antibody - BSA Free Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: DWDWDEGVGKLAKGYVWNGGSNPQANRQTSDSSSAKMSTPADKVLRKFENKINLDKLNVTDSVINKVTEKSRQKEADMYRIKD
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RIOK1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
RIOK1 Protein (NBP2-47301PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for RIOK1 Antibody - BSA Free

  • AD034
  • bA288G3.1
  • EC 2.7.11.1
  • FLJ30006
  • MGC12903
  • prematurely truncated due to insertion of intronic sequence
  • RIO kinase 1 (yeast)
  • RIO kinase 1
  • RRP10
  • serine/threonine-protein kinase RIO1

Background

RIO kinase 1 (RIOK1) is a member of the RIO family of atypical serine protein kinases first characterized in yeast. There are three RIO kinase subfamilies: RIO1, RIO2, and RIO3. RIOK1 and RIOK2 proteins are present in organisms from Archaea to humans. Studies of RIO kinases in yeast indicate that they play a role in ribosome biogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RIOK1