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RIOK1 Antibody

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Immunohistochemistry-Paraffin: RIOK1 Antibody [NBP2-47300] - Analysis in human testis and pancreas tissues using NBP2-47300 antibody. Corresponding RIOK1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: RIOK1 Antibody [NBP2-47300] - Analysis in human testis and pancreas tissues using NBP2-47300 antibody. Corresponding RIOK1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: RIOK1 Antibody [NBP2-47300] - Staining of human testis shows high expression.
Immunohistochemistry-Paraffin: RIOK1 Antibody [NBP2-47300] - Staining of human pancreas shows no cytoplasmic positivity in exocrine glandular cells as expected.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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RIOK1 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: NVNDFFMRHSVAVMTVRELFEFVTDPSITHENMDAYLSKAMEIASQRTKEERSSQDHVDEEVFKRAYIPRTLNEVKNYERDMDIIMKLKEEDMAMNAQQDNILYQTVTGLKKDLSGVQKVPALLENQVEERTCSDSEDIGSSEC
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RIOK1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
RIOK1 Protein (NBP2-47300PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%), Rat (84%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for RIOK1 Antibody

  • AD034
  • bA288G3.1
  • EC 2.7.11.1
  • FLJ30006
  • MGC12903
  • prematurely truncated due to insertion of intronic sequence
  • RIO kinase 1 (yeast)
  • RIO kinase 1
  • RRP10
  • serine/threonine-protein kinase RIO1

Background

RIO kinase 1 (RIOK1) is a member of the RIO family of atypical serine protein kinases first characterized in yeast. There are three RIO kinase subfamilies: RIO1, RIO2, and RIO3. RIOK1 and RIOK2 proteins are present in organisms from Archaea to humans. Studies of RIO kinases in yeast indicate that they play a role in ribosome biogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Flow Cytometry: c-Myc Antibody (9E10) [NB600-302] - An intracellular stain was performed on Jurkat cells with c-Myc Antibody (9E10) NB600-302R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.
Western Blot: c-Myc Antibody (9E10) [NB600-302] - CtIP is phosphorylated by CDKs at multiple sites. Myc-BRCA1 and/or HA-CtIP WT or indicated mutants were expressed in 293T cells and co-immunoprecipitation was performed. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pgen.1003277) licensed under a CC-BY licence.
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(RPSA/2699) - Azide a ...
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Immunohistochemistry-Paraffin: RPSA Antibody (RPSA/2699) - Azide and BSA Free [NBP2-79854] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with RPSA Antibody (RPSA/2699).
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Bioinformatics

Gene Symbol RIOK1