RIOK1 Antibody

Western Blot: RIOK1 Antibody [NBP1-30103] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IPloaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-RIOK1 antibody used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. RIOK1 was also immunoprecipitated by rabbit anti-RIOK1 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

(A) FP results for linear sequence 29 and2nd and3rd generation macrocyclic PAPIIs with PRMT5-MEP50 (n = 3). (B) FP competition of representative linear and macrocyclicpeptides with FITC-labeled 50 for binding to PRMT5-MEP50(n = 3). (C) FP competition of 2nd and 3rd generationPAPIIs and the unlabeled pICln protein with the Alexa 488-labeledfull-length human pICln protein for binding to PRMT5-MEP50 (n = 3). (D) Comparison of the effects of linear and cyclicpeptides on the melting temperature of the TIM-MEP50 complex, as determinedby TSA (n = 6). (E) Pull-down assay with peptides 55 and scrambled 56 immobilized on the DBCO beadsusing MCF7 cell lysate. Immobilized 55 was also testedwith the lysate containing 100 μM of free 56. (F)GFP-IP from the lysate of Flp-In T-REx 293-GFP and Flp-In T-REx 293-GFP-PRMT5overexpressing cells, testing compound 53 and scrambled 54 at 50 μM. (G) GFP-IP from Flp-In T-REx 293-GFP-pIClnand Flp-In T-Rex 293-GFP-RioK1 cytoplasmic extract, testing 53 and 54 at 50 μM. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36378254), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

(A) FP results for linear sequence 29 and2nd and3rd generation macrocyclic PAPIIs with PRMT5-MEP50 (n = 3). (B) FP competition of representative linear and macrocyclicpeptides with FITC-labeled 50 for binding to PRMT5-MEP50(n = 3). (C) FP competition of 2nd and 3rd generationPAPIIs and the unlabeled pICln protein with the Alexa 488-labeledfull-length human pICln protein for binding to PRMT5-MEP50 (n = 3). (D) Comparison of the effects of linear and cyclicpeptides on the melting temperature of the TIM-MEP50 complex, as determinedby TSA (n = 6). (E) Pull-down assay with peptides 55 and scrambled 56 immobilized on the DBCO beadsusing MCF7 cell lysate. Immobilized 55 was also testedwith the lysate containing 100 μM of free 56. (F)GFP-IP from the lysate of Flp-In T-REx 293-GFP and Flp-In T-REx 293-GFP-PRMT5overexpressing cells, testing compound 53 and scrambled 54 at 50 μM. (G) GFP-IP from Flp-In T-REx 293-GFP-pIClnand Flp-In T-Rex 293-GFP-RioK1 cytoplasmic extract, testing 53 and 54 at 50 μM. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36378254), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

RIOK1 Antibody Summary

• Immunogen: maps to a region between residue 1 and 50 of human RIO kinase 1 using the numbering given in entry NP_113668.2
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RIOK1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Western blot of lysates performed using standard western blot reagents and 4-20% SDS-PAGE.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RIOK1 Antibody

• AD034
• bA288G3.1
• EC 2.7.11.1
• FLJ30006
• MGC12903
• prematurely truncated due to insertion of intronic sequence
• RIO kinase 1 (yeast)
• RIO kinase 1
• RRP10
• serine/threonine-protein kinase RIO1

Background

RIO kinase 1 (RIOK1) is a member of the RIO family of atypical serine protein kinases first characterized in yeast. There are three RIO kinase subfamilies: RIO1, RIO2, and RIO3. RIOK1 and RIOK2 proteins are present in organisms from Archaea to humans. Studies of RIO kinases in yeast indicate that they play a role in ribosome biogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RIOK1 Antibody (NBP1-30103)(2)

Publications using NBP1-30103

• Krzyzanowski A, Esser LM, Willaume A et al. Development of Macrocyclic PRMT5-Adaptor Protein Interaction Inhibitors Journal of medicinal chemistry 2022-11-24 [PMID: 36378254] (WB, Human)
• Jan Cox, Lea Marie Esser, Maximilian Jüdt, Katharina Schmitz, Kaja Reiffert, Matthias Grimmler, Björn Stork, Sebastian Wesselborg, Christoph Peter NF90/NFAR (nuclear factors associated with dsRNA) - a new methylation substrate of the PRMT5-WD45-RioK1 complex. Biological chemistry 2022-09-13 [PMID: 36040368]

Bioinformatics

• Gene Symbol: RIOK1
• Entrez:
  • 83732(Human)
• Uniprot:
  • Q9BRS2(Human)