Rictor Antibody (454313)

Western blot shows lysates of HEK293 human embryonic kidney cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human Rictor Monoclonal Antibody (Catalog # MAB4598) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Rictor at approximately 200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 454313
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Rictor Antibody (454313) Summary

• Immunogen: E. coli-derived recombinant human Rictor
  Met1-Leu250
  Accession # Q6R327
• Specificity: Detects endogenous human Rictor in Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: RICTOR
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Rictor Antibody (454313)

• AVO3 homolog
• AVO3
• hAVO3
• KIAA1999DKFZp686B11164
• mAVO3
• MGC39830
• PIA
• Pianissimo
• rapamycin-insensitive companion of mTOR
• Rictor
• RPTOR independent companion of MTOR, complex 2
• TORC2-specific protein AVO3

Background

Rictor (rapamycin-insensitive companion of mTOR) is a member of the mTORC2 complex, which regulates cell growth by integrating stress, energy, and nutrient signals with growth factor derived signals. Rictor contains no obvious catalytic motifs, but knockdown studies of mTOR or Rictor results in a loss actin polymerization and cell spreading. In vitro, the mTOR-Rictor complex phosphorylates Akt/PKB on Ser473, an essential step for full activation of Akt/PKB. Additionally, recent studies have shown that mTOR-Rictor regulates PKC alpha phosphorylation and PDGFR beta expression and signaling. Rictor has also been described as a protein substrate for Akt.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RICTOR
• Uniprot:
  • Q6R327()