Rictor Antibody (1A5)

Western Blot: Rictor Antibody (1A5) [NBP3-32903] - Western blot analysis of Rictor on HepG2 cell lysates with Rabbit anti-Rictor antibody (NBP3-32903) at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 192 kDa Observed band size: 192 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32903) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: Rictor Antibody (1A5) [NBP3-32903] - Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Rictor antibody (NBP3-32903) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32903) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/ Immunofluorescence: Rictor Antibody (1A5) [NBP3-32903] - Immunocytochemistry analysis of A431 cells labeling Rictor with Rabbit anti-Rictor antibody (NBP3-32903) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Rictor antibody (NBP3-32903) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Flow Cytometry: Rictor Antibody (1A5) [NBP3-32903] - Flow cytometric analysis of Hela cells labeling Rictor. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32903, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Product Discontinued

Rictor Antibody (1A5) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein within human Rictor aa 1459-1708 / 1708. (Uniprot: Q6R327)
• Localization: Cytosol, TORC2 complex.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: RICTOR
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:500-1:1000
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:500
  • Western Blot 1:500
• Theoretical MW: 192 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.1% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Rictor Antibody (1A5)

• AVO3 homolog
• AVO3
• hAVO3
• KIAA1999DKFZp686B11164
• mAVO3
• MGC39830
• PIA
• Pianissimo
• rapamycin-insensitive companion of mTOR
• Rictor
• RPTOR independent companion of MTOR, complex 2
• TORC2-specific protein AVO3

Background

Rictor is a binding partner of mTOR. The rictor mTOR complex modulates the phosphorylation of Protein Kinase C alpha and the actin cytoskeleton.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RICTOR