Ribosomal Protein S6/RPS6 [p Ser244] Antibody - Azide Free

Western Blot: RPS6 [p Ser244] Antibody [NBP2-29653] - Jurkat cell lysate showing specific immunolabeling of the ~28k rpS6 phosphorylated at Ser244 (Control). Phosphospecificity is shown in the second lane where immunolabeling is blocked by preadsorption of the phospho-peptide used as antigen (peptide) but not by the corresponding dephosphopeptide (not shown).

• Reactivity: Hu, Mu, Rt, Ch, Gt, Gp, Pm, Xp, Ze
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

Ribosomal Protein S6/RPS6 [p Ser244] Antibody - Azide Free Summary

• Description: Novus Biologicals Rabbit Ribosomal Protein S6/RPS6 [p Ser244] Antibody - Azide Free (NBP2-29653) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser244 conjugated to KLH. Accession # P62754
• Modification: p Ser244
• Specificity: Specific for endogenous levels of the ~28 kDa Ribosomal Protein S6/RPS6 phosphorylated at Ser244. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.
• Preparation Method: Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
• Predicted Species: Rat (100%), Chicken (100%), Goat (100%), Xenopus (100%), Primate (100%), Zebrafish (100%), Guinea Pig (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RPS6
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 28 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for Ribosomal Protein S6/RPS6 [p Ser244] Antibody - Azide Free

• Phosphoprotein NP33, 40S ribosomal protein S6
• pS6
• Ribosomal Protein S6
• RPS6
• S6

Background

Ribosomal protein S6 (rpS6) is a critical component of the 40 S ribosomal subunit that mediates translation initiation at the 5'-m7GpppG cap of mRNA. The rpS6 protein is both cytoplasmic and nuclear localized (Chen and Dittmer 2011). In response to mitogenic stimuli, rpS6 undergoes ordered C-terminal phosphorylation by p70 S6 kinases and p90 ribosomal S6 kinases on four Ser residues (Ser-235, Ser-236, Ser-240, and Ser-244) whose modification potentiates rpS6 cap binding activity (Hutchinson et al., 2011). Additionally, rpS6 phosphorylation and function are highly regulated and have been implicated in the regulation of translational initiation and protein synthesis in response to extracellular stimuli such as TRAIL and gamma interferon (IFN-gamma), as well as upon activation of the phosphatidylinositol 3-kinase (PI3K)-Akt-mTOR pathway (Chen and Dittmer 2011).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RPS6
• Entrez:
  • 20104(Mouse)
• Uniprot:
  • P62754(Mouse)