HAF007). A specific band was detected for Ribosomal Protein S6 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." alt="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human/Mouse/Rat Ribosomal Protein S6 Monoclonal Antibody (Catalog # MAB5436) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Ribosomal Protein S6 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | IC5436G-100UG | |||
| Carrier Free | MAB5436-SP | |||
HAF016). A specific band was detected for Ribosomal Protein S6 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat Ribosomal Protein S6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5436) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Ribosomal Protein S6 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF5436-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | DYC3918E | |||
| Carrier Free | DYC3918-5 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-23531-20ul | |||
![Western Blot: Ribosomal Protein S6/RPS6 Antibody [NB100-1595] - Detection of human and mouse RPS6 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPS6 antibody NB100-1595 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.Previous](http://images.novusbio.com/fullsize/Ribosomal-Protein-S6-RPS6-Antibody-Western-Blot-NB100-1595-img0007.jpg "Western Blot: Ribosomal Protein S6/RPS6 Antibody [NB100-1595] - Detection of human and mouse RPS6 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPS6 antibody NB100-1595 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.Previous")
![Immunohistochemistry-Paraffin: Ribosomal Protein S6/RPS6 Antibody [NB100-1595] - Section of human lung carcinoma. Antibody: Affinity purified rabbit anti-RPS6 (NB100-1595). Detection: DAB](http://images.novusbio.com/fullsize/Ribosomal-Protein-S6-RPS6-Antibody-Immunohistochemistry-NB100-1595-img0009.jpg)
![Immunoprecipitation: Ribosomal Protein S6/RPS6 Antibody [NB100-1595] - Detection of human RPS6 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RPS6 antibody NB100-1595 (lot NB100-1595-2) used for IP at 6 ug per reaction. RPS6 was also immunoprecipitated by a previous lot of this antibody (lot NB100-1595-1). For blotting immunoprecipitated RPS6, NB100-1595 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/Ribosomal-Protein-S6-RPS6-Antibody-Immunoprecipitation-NB100-1595-img0008.jpg)
![Western Blot: Ribosomal Protein S6/RPS6 Antibody [NB100-1595] - Loss of SPAG5 & CLUH lead to hyperactivation of mTORC1 signaling.(A, B) MSigDB Hallmark pathways of downregulated (E) or upregulated (F) proteins (with a cutoff of p ≤ 0.05; q ≤ 0.15) detected in proteomics analysis of SPAG5 ind-KO cells (Figure 4A, Supplementary file 3) using the EnrichR webtool. (C) Western blots of WT & SPAG5 ind-KO HeLa cells transfected with siRNA against CLUH or untargeted control siRNA. Cells were induced for 4 days with doxycycline, additionally downregulated for the last 3 days & grown for the last 8 hr in basal or HBSS media without doxycycline. Pan-actin was used as loading control. (D) Quantification of experiments as shown in C (n = 4 independent experiments). Antibody signal was normalized to pan-actin signal, & signal of phospho-protein was normalized to signal of the total protein. Bars show mean ± standard error of the mean (SEM) & dots represent values of individual replicates. One-way analysis of variance (ANOVA) with post hoc Tukey’s multiple comparison tests were performed with *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001.Figure 5—figure supplement 1—source data 1.Uncropped blots for Figure 5—figure supplement 1C.Figure 5—figure supplement 1—source data 2.Unedited blots for Figure 5—figure supplement 1C.Uncropped blots for Figure 5—figure supplement 1C.Unedited blots for Figure 5—figure supplement 1C. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35559794), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1595_rabbit-polyclonal-ribosomal-protein-s6-rps6-antibody-31020241683913.jpg)
![L1 bodies (LBs) are large cytoplasmic compartments enriched in ORF1p, L1 mRNA, and ribosomes.(A–E) Electron micrographs exemplifying the formation of LBs in Mael-/- spermatocytes. Boxed areas in A and B identify small cytoplasmic aggregates magnified in A’ and B’, respectively. Boxed area in C identifies an intermediate size LB magnified in C’. D and E show more prominent LBs, partially surrounded by a bilayer membrane (red arrows). Nuc: nucleus. Cyto: cytoplasm. NE: nuclear envelope. (F) Immunofluorescence staining of L1 ORF1p on Mael-/- spermatocytes shows ORF1p accumulation in LBs (left panel, magenta). 3D reconstruction of LBs (magenta) and nuclei (blue) (middle and right panels); numbers [1–5] are used to label corresponding cells. Scale bars: 5 μm. (G) HCR RNA-FISH of L1 RNA (green) and immunofluorescence staining of L1 ORF1p (magenta) on Mael+/- and Mael-/- testis sections. Scale bars: 5 μm. (H) Double immunofluorescence staining of ribosomal protein S6 (RPS6, top two rows, green) or L28 (RPL28, bottom two rows, green) and L1 ORF1p (magenta) showing their colocalization in LBs. Dotted white lines mark seminiferous tubule boundaries. Scale bars: 15 μm. See also S1 Fig. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37307272), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1595_rabbit-polyclonal-ribosomal-protein-s6-rps6-antibody-immunocytochemistry-immunofluorescence-13220261739388.jpg)
![Western Blot: Ribosomal Protein S6/RPS6 Antibody (A6B9-R) [NBP3-32918] - Western blot analysis of Ribosomal Protein S6/RPS6 on different lysates with Mouse anti-Ribosomal Protein S6/RPS6 antibody (NBP3-32918) at 1/1,000 dilution.<br/><br/> Lane 1: HeLa cell lysate (20 ug/Lane)<br/> Lane 2: MCF7 cell lysate (20 ug/Lane)<br/> Lane 3: HepG2 cell lysate (20 ug/Lane)<br/> Lane 4: HEK-293 cell lysate (20 ug/Lane)<br/> Lane 5: HCT 116 cell lysate (20 ug/Lane)<br/> Lane 6: THP-1 cell lysate (20 ug/Lane)<br/> Lane 7: NIH/3T3 cell lysate (20 ug/Lane)<br/> Lane 8: PC-12 cell lysate (20 ug/Lane)<br/> Lane 9: Human kidney tissue lysate (40 ug/Lane)<br/> Lane 10: Mouse spleen tissue lysate (40 ug/Lane)<br/><br/> Predicted band size: 29 kDa<br/> Observed band size: 33 kDa<br/><br/> Exposure time: 3 minutes;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32918) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32918_mouse-ribosomal-protein-s6-rps6-mab-a6b9-r-288202412461611.jpg "Western Blot: Ribosomal Protein S6/RPS6 Antibody (A6B9-R) [NBP3-32918] - Western blot analysis of Ribosomal Protein S6/RPS6 on different lysates with Mouse anti-Ribosomal Protein S6/RPS6 antibody (NBP3-32918) at 1/1,000 dilution.<br/><br/> Lane 1: HeLa cell lysate (20 ug/Lane)<br/> Lane 2: MCF7 cell lysate (20 ug/Lane)<br/> Lane 3: HepG2 cell lysate (20 ug/Lane)<br/> Lane 4: HEK-293 cell lysate (20 ug/Lane)<br/> Lane 5: HCT 116 cell lysate (20 ug/Lane)<br/> Lane 6: THP-1 cell lysate (20 ug/Lane)<br/> Lane 7: NIH/3T3 cell lysate (20 ug/Lane)<br/> Lane 8: PC-12 cell lysate (20 ug/Lane)<br/> Lane 9: Human kidney tissue lysate (40 ug/Lane)<br/> Lane 10: Mouse spleen tissue lysate (40 ug/Lane)<br/><br/> Predicted band size: 29 kDa<br/> Observed band size: 33 kDa<br/><br/> Exposure time: 3 minutes;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32918) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Ribosomal Protein S6/RPS6 Antibody (A6B9-R) [NBP3-32918] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Mouse anti-Ribosomal Protein S6/RPS6 antibody (NBP3-32918) at 1/500 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32918) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32918_mouse-ribosomal-protein-s6-rps6-mab-a6b9-r-29820241492655.jpg)
![Immunocytochemistry/ Immunofluorescence: Ribosomal Protein S6/RPS6 Antibody (A6B9-R) [NBP3-32918] - Immunocytochemistry analysis of HeLa cells labeling Ribosomal Protein S6/RPS6 with Mouse anti-Ribosomal Protein S6/RPS6 antibody (NBP3-32918) at 1/100 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Ribosomal Protein S6/RPS6 antibody (NBP3-32918) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32918_mouse-ribosomal-protein-s6-rps6-mab-a6b9-r-29202410234256.jpg)
![Flow Cytometry: Ribosomal Protein S6/RPS6 Antibody (A6B9-R) [NBP3-32918] - Flow cytometric analysis of HeLa cells labeling Ribosomal Protein S6/RPS6.<br/><br/> Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32918, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32918_mouse-ribosomal-protein-s6-rps6-mab-a6b9-r-392024884653.jpg)
NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for ![Western Blot: Ribosomal Protein S6/RPS6 [p Ser235, p Ser236] Antibody (PS01-04) [NBP3-32796] - Western blot analysis of Ribosomal Protein S6/RPS6 on different lysates with Rabbit anti-Ribosomal Protein S6/RPS6 antibody (NBP3-32796) at 1/1,000 dilution.<br/><br/> Lane 1: NIH/3T3 cell lysate<br/> Lane 2: THP-1 cell lysate<br/> Lane 3: Hela cell lysate<br/><br/> Lysates/proteins at 10 ug/Lane.<br/><br/> Predicted band size: 29 kDa<br/> Observed band size: 33 kDa<br/><br/> Exposure time: 30 seconds;<br/><br/> 12% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32796) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32796_rabbit-ribosomal-protein-s6-rps6-p-ser235-p-ser236-mab-28820241246165.jpg "Western Blot: Ribosomal Protein S6/RPS6 [p Ser235, p Ser236] Antibody (PS01-04) [NBP3-32796] - Western blot analysis of Ribosomal Protein S6/RPS6 on different lysates with Rabbit anti-Ribosomal Protein S6/RPS6 antibody (NBP3-32796) at 1/1,000 dilution.<br/><br/> Lane 1: NIH/3T3 cell lysate<br/> Lane 2: THP-1 cell lysate<br/> Lane 3: Hela cell lysate<br/><br/> Lysates/proteins at 10 ug/Lane.<br/><br/> Predicted band size: 29 kDa<br/> Observed band size: 33 kDa<br/><br/> Exposure time: 30 seconds;<br/><br/> 12% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32796) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Ribosomal Protein S6/RPS6 [p Ser235, p Ser236] Antibody (PS01-04) [NBP3-32796] - Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-Ribosomal Protein S6/RPS6 antibody (NBP3-32796) at 1/200 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32796) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32796_rabbit-ribosomal-protein-s6-rps6-p-ser235-p-ser236-mab-2982024149269.jpg)
![Immunocytochemistry/ Immunofluorescence: Ribosomal Protein S6/RPS6 [p Ser235, p Ser236] Antibody (PS01-04) [NBP3-32796] - Immunocytochemistry analysis of Hela cells labeling Ribosomal Protein S6/RPS6 with Rabbit anti-Ribosomal Protein S6/RPS6 antibody (NBP3-32796) at 1/50 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Ribosomal Protein S6/RPS6 antibody (NBP3-32796) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32796_rabbit-ribosomal-protein-s6-rps6-p-ser235-p-ser236-mab-29202410484848.jpg)
![Western Blot: Ribosomal Protein S6/RPS6 [p Ser240, p Ser244] Antibody (PS00-65) [NBP3-32797] - Western blot analysis of Ribosomal Protein S6/RPS6 on A431 cell lysates with Rabbit anti-Ribosomal Protein S6/RPS6 antibody (NBP3-32797) at 1/1,000 dilution.<br/><br/> Lysates/proteins at 10 ug/Lane.<br/><br/> Predicted band size: 29 kDa<br/> Observed band size: 33 kDa<br/><br/> Exposure time: 2 minutes;<br/><br/> 12% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32797) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32797_rabbit-ribosomal-protein-s6-rps6-p-ser240-p-ser244-mab-288202412442945.jpg "Western Blot: Ribosomal Protein S6/RPS6 [p Ser240, p Ser244] Antibody (PS00-65) [NBP3-32797] - Western blot analysis of Ribosomal Protein S6/RPS6 on A431 cell lysates with Rabbit anti-Ribosomal Protein S6/RPS6 antibody (NBP3-32797) at 1/1,000 dilution.<br/><br/> Lysates/proteins at 10 ug/Lane.<br/><br/> Predicted band size: 29 kDa<br/> Observed band size: 33 kDa<br/><br/> Exposure time: 2 minutes;<br/><br/> 12% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32797) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Ribosomal Protein S6/RPS6 [p Ser240, p Ser244] Antibody (PS00-65) [NBP3-32797] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Ribosomal Protein S6/RPS6 antibody (NBP3-32797) at 1/5,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32797) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32797_rabbit-ribosomal-protein-s6-rps6-p-ser240-p-ser244-mab-29820241492617.jpg)
![Immunocytochemistry/ Immunofluorescence: Ribosomal Protein S6/RPS6 [p Ser240, p Ser244] Antibody (PS00-65) [NBP3-32797] - Immunocytochemistry analysis of SK-Br-3 cells labeling Ribosomal Protein S6/RPS6 with Rabbit anti-Ribosomal Protein S6/RPS6 antibody (NBP3-32797) at 1/50 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Ribosomal Protein S6/RPS6 antibody (NBP3-32797) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32797_rabbit-ribosomal-protein-s6-rps6-p-ser240-p-ser244-mab-29202410234217.jpg)
![Western Blot: Ribosomal Protein S6/RPS6 Antibody [NBP1-87099] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: MOLT-4](http://images.novusbio.com/fullsize/Ribosomal-Protein-S6-RPS6-Antibody-Western-Blot-NBP1-87099-img0004.jpg "Western Blot: Ribosomal Protein S6/RPS6 Antibody [NBP1-87099] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: MOLT-4")
![Immunocytochemistry/Immunofluorescence: Ribosomal Protein S6/RPS6 Antibody [NBP1-87099] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol & endoplasmic reticulum.](http://images.novusbio.com/fullsize/Ribosomal-Protein-S6-RPS6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87099-img0006.jpg)
![Western Blot: Ribosomal Protein S6/RPS6 Antibody (4C7P4) [NBP3-15435] - Western blot analysis of extracts of various cell lines, using Ribosomal Protein S6/RPS6 antibody (NBP3-15435) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Ribosomal-Protein-S6-RPS6-Antibody-4C7P4-Western-Blot-NBP3-15435-img0001.jpg "Western Blot: Ribosomal Protein S6/RPS6 Antibody (4C7P4) [NBP3-15435] - Western blot analysis of extracts of various cell lines, using Ribosomal Protein S6/RPS6 antibody (NBP3-15435) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![ICC/IF: Ribosomal Protein S6/RPS6 Antibody (4C7P4) [NBP3-15435] -Confocal imaging of NIH/3T3 cells using S6 Ribosomal Protein (RPS6) Rabbit mAb (dilution 1:100)(Red). The cells were counterstained with alpha -Tubulin Mouse mAb (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15435_rabbit-ribosomal-protein-s6-rps6-mab-4c7p4-immunocytochemistry-immunofluorescence-16120251042..png)
![ICC/IF: Ribosomal Protein S6/RPS6 Antibody (4C7P4) [NBP3-15435] -Confocal imaging of C6 cells using S6 Ribosomal Protein (RPS6) Rabbit mAb (dilution 1:100)(Red). The cells were counterstained with alpha -Tubulin Mouse mAb (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15435_rabbit-ribosomal-protein-s6-rps6-mab-4c7p4-immunocytochemistry-immunofluorescence-161202510555..png)
![Immunocytochemistry/ Immunofluorescence: Ribosomal Protein S6/RPS6 Antibody (4C7P4) [Ribosomal Protein S6/RPS6] - Confocal imaging of C6 cells using Ribosomal Protein S6/RPS6 Rabbit mAb . The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15435_rabbit-ribosomal-protein-s6-rps6-mab-4c7p4-52202517152772.jpg)
![ELISA Ribosomal Protein S6/RPS6 [p Ser235, p Ser236] [Biotin]](http://images.novusbio.com/fullsize/dyc3918-2_phospho-rps6-s235-s236-duoset-ic-elisa-2-plate-elisa-169202094547.jpg "ELISA Ribosomal Protein S6/RPS6 [p Ser235, p Ser236] [Biotin]")
![Western Blot: Ribosomal Protein S6/RPS6 [p Ser240, p Ser244] Antibody (0018) [NBP3-23531] - Western blot analysis of extracts from serum-starved HEK293, treated with 20% FBS (30 min), using NBP3-23531 at dilution of 1:1000, 1:5000 and 1:25000.](http://images.novusbio.com/fullsize/nbp3-23531_rabbit-ribosomal-protein-s6-rps6-mab-0018-azide-bsa-free-235202511354146.png "Western Blot: Ribosomal Protein S6/RPS6 [p Ser240, p Ser244] Antibody (0018) [NBP3-23531] - Western blot analysis of extracts from serum-starved HEK293, treated with 20% FBS (30 min), using NBP3-23531 at dilution of 1:1000, 1:5000 and 1:25000.")
![Western Blot: Ribosomal Protein S6/RPS6 [p Ser240, p Ser244] Antibody (0018) [NBP3-23531] - Western blot analysis of extracts from serum-starved , untreated (line A); treated with FBS (20%, 30min), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using NBP3-23531 at 1:5000 dilution.](http://images.novusbio.com/fullsize/nbp3-23531_rabbit-ribosomal-protein-s6-rps6-mab-0018-azide-bsa-free-235202521451756.png)
![Immunohistochemistry-Paraffin: Ribosomal Protein S6/RPS6 [p Ser240, p Ser244] Antibody (0018) [NBP3-23531] - Immunohistochemical analysis of paraffin-embedded human carcinoma of sigmoid tissue,untreated (left) or lambda phosphatase-treated (right), using NBP3-23531 at 1:2000 dilution.](http://images.novusbio.com/fullsize/nbp3-23531_rabbit-ribosomal-protein-s6-rps6-mab-0018-azide-bsa-free-235202514492317.png)
![Western Blot: Ribosomal Protein S6/RPS6 [p Ser240, p Ser244] Antibody (0018) [NBP3-23531] - Western blot analysis of extracts from serum-starved HEK293, untreated (line A); treated with FBS (20%, 30min) (line B); treated with FBS and -phosphatase (line C) using NBP3-23531 at 1:5000 dilution.](http://images.novusbio.com/fullsize/nbp3-23531_rabbit-ribosomal-protein-s6-rps6-mab-0018-azide-bsa-free-235202512205527.png)
![Western Blot: Ribosomal Protein S6/RPS6 [p Ser240, p Ser244] Antibody [NBP3-13315] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ribosomal Protein S6/RPS6 (phospho Ser240/Ser244) antibody (NBP3-13315) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Ribosomal-Protein-S6-RPS6-[p-Ser240--p-Ser244]-Antibody-Western-Blot-NBP3-13315-img0002.jpg "Western Blot: Ribosomal Protein S6/RPS6 [p Ser240, p Ser244] Antibody [NBP3-13315] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ribosomal Protein S6/RPS6 (phospho Ser240/Ser244) antibody (NBP3-13315) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Western Blot: Ribosomal Protein S6/RPS6 [p Ser240, p Ser244] Antibody [NBP3-13315] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ribosomal Protein S6/RPS6 (phospho Ser240/Ser244) antibody (NBP3-13315) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Ribosomal-Protein-S6-RPS6-[p-Ser240--p-Ser244]-Antibody-Western-Blot-NBP3-13315-img0001.jpg)
![Western Blot: Ribosomal Protein S6/RPS6 [p Ser235] Antibody [NBP3-13078] - Untreated (-) and treated (+) 293T whole cell extracts starvation for 16 hrs (40 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ribosomal Protein S6/RPS6 (phospho Ser235) antibody (NBP3-13078) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Ribosomal-Protein-S6-RPS6-[p-Ser235]-Antibody-Western-Blot-NBP3-13078-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: Ribosomal Protein S6/RPS6 [p Ser235] Antibody [NBP3-13078] - Untreated (-) and treated (+) 293T whole cell extracts starvation for 16 hrs (40 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ribosomal Protein S6/RPS6 (phospho Ser235) antibody (NBP3-13078) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Ribosomal Protein S6/RPS6 [p Ser235] Antibody [NBP3-13078] - Ribosomal Protein S6/RPS6 (phospho Ser235) antibody detects Ribosomal Protein S6/RPS6 (phospho Ser235) protein at cytoplasm and nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Ribosomal Protein S6/RPS6 (phospho Ser235) antibody (NBP3-13078) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Ribosomal-Protein-S6-RPS6-[p-Ser235]-Antibody-Immunohistochemistry-Paraffin-NBP3-13078-img0001.jpg)
