Recombinant E. coli Ribonuclease Inhibitor Protein

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Product Details

Summary
Reactivity EcSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

Order Details

Recombinant E. coli Ribonuclease Inhibitor Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-155 of E.coli Ribonuclease Inhibitor

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMLKQVEI FTDGSCLGNP GPGGYGAILR YRGREKTFSA GYTRTTNNRM ELMAAIVALE ALKEHCEVIL STDSQYVRQG ITQWIHNWKK RGWKTADKKP VKNVDLWQRL DAALGQHQIK WEWVKGHAGH PENERCDELA RAAAMNPTLE DTGYQVEV

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
RNH1
Purity
>95% pure by SDS-PAGE

Applications/Dilutions

Theoretical MW
20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 2mM DTT
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>95% pure by SDS-PAGE

Alternate Names for Recombinant E. coli Ribonuclease Inhibitor Protein

  • Placental ribonuclease inhibitor
  • Placental RNase inhibitor
  • PRI
  • RAIMGC4569
  • ribonuclease inhibitor
  • ribonuclease/angiogenin inhibitor 1MGC18200
  • ribonuclease/angiogenin inhibitor
  • RNHMGC54054

Background

rnhA is an endonuclease that specifically degrades the RNA of RNA-DNA hybrids. Localized to the nucleus, this protein mediates the removal of Okazaki fragment RNA primers that are present on the lagging strand during DNA replication. rnhA catalyzes the endonucleolytic cleavage of RNA to a 5'-phosphomonoester and is able to bind magnesium or manganese as cofactors. Recombinant e.coli rnhA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol RNH1