Ribonuclease A Antibody Pair [HRP]

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Sandwich ELISA: Ribonuclease A Antibody Pair [HRP] [NBP2-79414] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, S-ELISA
Conjugate
HRP
Standard Curve Range
62.5-4000 pg/ml
Sensitivity
62.5 pg/ml

Order Details

Ribonuclease A Antibody Pair [HRP] Summary

Description
Solid Phase sandwich ELISA for the quantitative determination of Human Ribonuclease A.
Specificity
This solid phase sandwich ELISA utilizes an antibody specific for Human Ribonuclease A.
Standard Curve Range
62.5-4000 pg/ml
Sensitivity
62.5 pg/ml
Assay Type
Sandwich ELISA
Sample Volume
100 ul
Gene
RNASE1

Applications/Dilutions

Application Notes
1. Capture Antibody: Dilute to a working concentration of 1 ug/ml in PBS before coating.
2. Detection Antibody: Dilute to working concentration of 0.25 ug/ml in detection antibody dilution buffer before use.
3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20C to -80C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 4000 pg/ml is recommended.

Packaging, Storage & Formulations

Storage
Storage is content dependent.

Kit Components

Components
  1. Detection Antibody: 0.2 mg/ml of rabbit anti-Human Ribonuclease A polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
  2. Capture Antibody: 1 mg/ml of mouse anti-Human Ribonuclease A monoclonal antibody (in PBS, pH 7.4).
  3. Standard: Each vial contains 85 ng of recombinant Human Ribonuclease A.

Alternate Names for Ribonuclease A Antibody Pair [HRP]

  • EC 3.1.27.5
  • HP-RNase
  • MGC12408
  • RIB1
  • RIB-1
  • Ribonuclease 1
  • Ribonuclease A
  • ribonuclease pancreatic
  • ribonuclease, RNase A family, 1 (pancreatic)
  • RNase A
  • RNase UpI-1
  • RNS1RNase 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are guaranteed for 6 months from date of receipt.

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Other Available Formats

HRP NBP2-79414

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Bioinformatics

Gene Symbol RNASE1