RHOT2 Antibody

Images

 
Western Blot: RHOT2 Antibody [H00089941-B01P] - Analysis of RHOT2 expression in transfected 293T cell line by RHOT2 polyclonal antibody. Lane 1: RHOT2 transfected lysate(67.98 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: RHOT2 Antibody [H00089941-B01P] - Human endometrial stromal cells stained with RHOT2 antibody (green). Image from verified customer review.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

RHOT2 Antibody Summary

Immunogen
RHOT2 (NP_620124.1, 1 a.a. ~ 618 a.a) full-length human protein.MRRDVRILLLGEAQVGKTSLILSLVGEEFPEEVPPRAEEITIPADVTPEKVPTHIVDYSEAEQTDEELREEIHKANVVCVVYDVSEEATIEKIRTKWIPLVNGGTTQGPRVPIILVGNKSDLRSGSSMEAVLPIMSQFPEIETCVECSAKNLRNISELFYYAQKAVLHPTAPLYDPEAKQLRPACAQALTRIFRLSDQDLDQALSDEELNAFQKSCFGHPLAPQALEDVKTVVCRNVAGGVREDRLTLDGFLFLN
Specificity
RHOT2 - ras homolog gene family, member T2,
Isotype
IgG
Clonality
Polyclonal
Host
Mouse
Gene
RHOT2
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA
Application Notes
Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot and also on transfected lysate in western blot. GST tag alone is used as a negative control.
Reviewed Applications
Read 1 Review rated 5
using
H00089941-B01P in the following applications:

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for RHOT2 Antibody

  • ARHT2
  • C16orf39
  • chromosome 16 open reading frame 39
  • EC 3.6.5
  • EC 3.6.5.-
  • hMiro-2
  • MIRO-2mitochondrial Rho 2
  • mitochondrial Rho GTPase 2
  • Ras homolog gene family member T2
  • ras homolog gene family, member T2
  • RASL

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RHOT2 Antibody (H00089941-B01P) (0)

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Review for RHOT2 Antibody (H00089941-B01P) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: IF.

Reviews using H00089941-B01P:
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IF
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Immunofluorescence RHOT2 H00089941-B01P
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reviewed by:
Gareth Healey
IF Human 01/18/2016
View

Summary

ApplicationImmunofluorescence
Sample TestedHuman endometrial stromal cells
SpeciesHuman

Blocking

Blocking Details3% BSA in PBS, 1 hour at room temperature

Primary Anitbody

Dilution Ratio1 in 200 in 3% BSA (in PBS), 2 hours at room temp

Secondary Antibody

Secondary DescriptionGoat anti-mouse IgG, AlexFluor 488
Secondary Manufacturer Cat#A11001
Secondary Concentration1 in 1000 dilution in 3% BSA

Details

Detection NotesConfocal microscopy, Zeiss LSM 710, Objective: 63x/1.40 Oil DIC M27, neg conts = unstained, primary only, secondary only
Fixation Details4% PFA for 20 min at 4C

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for RHOT2 Antibody (H00089941-B01P) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for RHOT2 Antibody (H00089941-B01P)

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Gareth Healey
01/18/2016
Application: IF
Species: Human

Bioinformatics

Gene Symbol RHOT2
Entrez
Uniprot