Rhot1 Antibody (CL1083) - BSA Free

Western Blot: Rhot1 Antibody (CL1083) [NBP2-52964] - Lane 1: Marker [kDa], Lane 2: Human cerebral cortex

Immunohistochemistry-Paraffin: Rhot1 Antibody (CL1083) [NBP2-52964] - Staining of human skeletal muscle shows weak cytoplasmic positivity in striated muscle fibers.

Immunohistochemistry-Paraffin: Rhot1 Antibody (CL1083) [NBP2-52964] - Staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neuronal cells, as well as positivity in neuropil.

Immunohistochemistry-Paraffin: Rhot1 Antibody (CL1083) [NBP2-52964] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.

Immunohistochemistry-Paraffin: Rhot1 Antibody (CL1083) [NBP2-52964] - Staining of human heart muscle shows strong cytoplasmic positivity in cardiomyocytes.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: CL1083
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Rhot1 Antibody (CL1083) - BSA Free Summary

• Description: Novus Biologicals Mouse Rhot1 Antibody (CL1083) - BSA Free (NBP2-52964) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed using a recombinant protein derived from Q8IXI2, with the exact immunogen sequence remaining proprietary.
• Predicted Species: Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: RHOT1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  Rhot1 Recombinant Protein Antigen (NBP2-52964PEP)

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for Rhot1 Antibody (CL1083) - BSA Free

• ARHT1
• EC 3.6.5
• EC 3.6.5.-
• FLJ11040
• FLJ12633
• hMiro-1
• Miro1
• MIRO-1mitochondrial Rho GTPase 1
• mitochondrial Rho 1
• rac-GTP binding protein-like protein
• Rac-GTP-binding protein-like protein
• Ras homolog gene family member T1
• ras homolog gene family, member T1
• Rhot1

Background

Mitochondrial GTPase involved in mitochondrial trafficking. Probably involved in control of anterograde transport of mitochondria and their subcellular distribution

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RHOT1