Rhophilin 2 Antibody

Immunohistochemistry-Paraffin: Rhophilin 2 Antibody [NB600-792] (5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Rhophilin 2 Antibody Summary

• Description: Novus Biologicals Goat Rhophilin 2 Antibody (NB600-792) is a polyclonal antibody validated for use in IHC and ELISA. Anti-Rhophilin 2 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-TNKNRQKSAST corresponding to internal region according to NP_149094.3.
• Predicted Species: Mouse (100%), Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: RHPN2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5 - 10 ug/ml
  • Immunohistochemistry-Paraffin 5 - 10 ug/ml
  • Peptide ELISA Detection limit 1:32000
• Application Notes: WB: Preliminary experiments gave an approx. 35 kDa band in human pancreas and prostate lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 77 kDa band according to NP_149094.2. The 35 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human kidney shows staining of cellular membrane and cytoplasm in convoluted tubular cells.
• Control:

  Rhophilin 2 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Rhophilin 2 Antibody

• GTP-Rho-binding protein 2
• P76RBE
• RHOBP
• rhophilin 2,76 kDa RhoB effector protein
• rhophilin, Rho GTPase binding protein 2
• rhophilin-2
• rhophilin-like Rho-GTPase binding protein

Background

The rho GTPase is involved in a signaling pathway that regulates cell adhesion and cytokinesis, among other activities.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Rhophilin 2 Antibody (NB600-792)(2)

Publications using NB600-792

• Yu F, Qiao P, Yin G et al. RHPN2 Promotes Malignant Cell Behaviours in Ovarian Cancer by Activating STAT3 Signalling J Neurosci Methods 2020-11-12 [PMID: 33204106]
• Peck JW, Oberst M, Bouker KB et al. The RhoA-binding protein, rhophilin-2, regulates actin cytoskeleton organization. J Biol Chem 2002-11-15 [PMID: 12221077]

Bioinformatics

• Gene Symbol: RHPN2
• Entrez:
  • 85415(Human)
  • 52428(Mouse)
  • 308516(Rat)
• Uniprot:
  • NP_149094.2(Human)