Rhophilin 2 Antibody

Images

 
Immunohistochemistry-Paraffin: Rhophilin 2 Antibody [NB600-792] (5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Rhophilin 2 Antibody Summary

Immunogen
Peptide with sequence C-TNKNRQKSAST corresponding to internal region according to NP_149094.3.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
RHPN2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5 - 10 ug/ml
  • Immunohistochemistry-Paraffin 5 - 10 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Preliminary experiments gave an approx. 35 kDa band in human pancreas and prostate lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 77 kDa band according to NP_149094.2. The 35 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human kidney shows staining of cellular membrane and cytoplasm in convoluted tubular cells.
Positive Control
Rhophilin 2 Lysate (NBL1-15364)
Publications
Read Publication using NB600-792.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Rhophilin 2 Antibody

  • GTP-Rho-binding protein 2
  • P76RBE
  • RHOBP
  • rhophilin 2,76 kDa RhoB effector protein
  • rhophilin, Rho GTPase binding protein 2
  • rhophilin-2
  • rhophilin-like Rho-GTPase binding protein

Background

The rho GTPase is involved in a signaling pathway that regulates cell adhesion and cytokinesis, among other activities.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Rhophilin 2 Antibody (NB600-792)(1)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RHPN2
Entrez
Uniprot