Recombinant Human RhoA Protein

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SDS-Page: Recombinant Human RhoA Protein [NBP1-50933] - 15 % SDS-PAGE (3ug)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

Order Details

Recombinant Human RhoA Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-190 of Human RhoA

Source: E.coli

Amino Acid Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMAA IRKKLVIVGD GACGKTCLLI VFSKDQFPEV YVPTVFENYV ADIEVDGKQV ELALWDTAGQ EDYDRLRPLS YPDTDVILMC FSIDSPDSLE NIPEKWTPEV KHFCPNVPII LVGNKKDLRN DEHTRRELAK MKQEPVKPEE GRDMANRIGA FGYMECSAKT KDGVREVFEM ATRAALQARR GKKKSGC

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
RHOA
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
25.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP1-50933.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human RhoA Protein

  • h12
  • oncogene RHO H12
  • ras homolog gene family, member A
  • Rho cDNA clone 12
  • RHO12
  • RhoA
  • RHOH12ARH12ARHAAplysia ras-related homolog 12
  • small GTP binding protein RhoA
  • transforming protein RhoA

Background

RhoA belongs to the small GTPase superfamily, Rho family, proteins involved in the regulation and timing of cell division. This protein is a small GTPase protein to regulate the actin cytoskeleton in the formation of stress fibers and acts upon two known effector proteins: ROCK1 (Rho-associated, coiled-coil containing protein kinase 1) and DIAPH1 (diaphanous homolog 1 (Drosophila). Recombinant human RhoA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Publications for RhoA Protein (NBP1-50933)(1)

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HIF-1 beta - activating gene transcription in response to hypoxia
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A good helper on validating your FLOW and IHC data - Rabbit IgG Isotype Control
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Bioinformatics

Gene Symbol RHOA