RFX5 Antibody

Product Discontinued

RFX5 Antibody Summary

• Description: This antibody was prepared from monospecific antiserum by delipidation, salt fractionation and ion exchange chromatography.
• Immunogen: RFX5 (N-terminal specific) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
• Specificity: Anti-RFX5 (N-terminal specific) may react with unknown minor bands at 55 kDa and 85 kDa.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RFX5
• Purity: Ion exchange chromatography

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:25000
  • Gel Super Shift Assays
  • Western Blot 1:500-1:3000
• Application Notes: This product was assayed by immunoblot and found to be reactive against RFX5 from a variety of fibroblast and B-cell lysates at a dilution of 1:500 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] Anti-RFX5 is suitable for the detection by immunoblot of human RFX5 a shows a 75 kDa band. This product was also tested in a gel supershift assay and found to be reactive against RFX5 complexes using 0.5 - 1.0 ul per assay. This product was assayed by immunoblot and found to be reactive against RFX5 from a variety of fibroblast and B-cell lysates at a dilution of 1:500 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] Anti-RFX5 is suitable for the detection by immunoblot of human RFX5 a shows a 75 kDa band. This product was also tested in a gel supershift assay and found to be reactive against RFX5 complexes using 0.5 - 1.0 ul per assay.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Ion exchange chromatography

Alternate Names for RFX5 Antibody

• DNA-binding protein RFX5
• Regulatory factor X 5
• regulatory factor X, 5 (influences HLA class II expression)

Background

RFX5 is the regulatory gene responsible for human MHC class II deficiency. Patients with this disease exhibit a characteristic defect in the binding of a nuclear factor, RFX, to the X box motif of MHC class II promoters. RFX5 is the 75 kDa subunit of the RFX complex which is absolutely essential and highly specific for regulating MHC class II gene expression and control of the immune response. RFX5 is a protein consisting of 616 amino acids.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for RFX5 Antibody (NBP1-77821). (Showing 1 - 1 of 1 FAQ).

1. I am planning to use this antibody for flow cytometry application. Can you make in conjugated format with either PE or Alexa 647?
   • NBP1-77821 has not yet been validated in Flow Cytometry, and we cannot guarantee performance of the antibody in an application that has not yet been validated. That being said, if you are interested in purchasing this antibody for use in flow, we are able to perform a custom conjugation.

Bioinformatics

• Gene Symbol: RFX5