RFX5 Antibody

Product Discontinued

RFX5 Antibody Summary

• Description: This antibody was prepared by repeated immunizations of an RFX5 peptide conjugate and purified as monospecific antiserum after delipidation and defibrination.
• Immunogen: RFX5 peptide corresponding to amino acids 320 to 494 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
• Isotype: Serum
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RFX5
• Purity: Delipidation and Defibrination

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:25000
  • Western Blot 1:500-1:3000
• Application Notes: This product was also tested in a gel supershift assay and found - be reactive against RFX5 complexes using 0.5 - 1.0 ul per assay. This product was also tested in a gel supershift assay and found - be reactive against RFX5 complexes using 0.5 - 1.0 ul per assay.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Delipidation and Defibrination

Alternate Names for RFX5 Antibody

• DNA-binding protein RFX5
• Regulatory factor X 5
• regulatory factor X, 5 (influences HLA class II expression)

Background

RFX5 is the regulatory gene responsible for human MHC class II deficiency. Patients with this disease exhibit a characteristic defect in the binding of a nuclear factor, RFX, to the X box motif of MHC class II promoters. RFX5 is the 75 kDa subunit of the RFX complex which is absolutely essential and highly specific for regulating MHC class II gene expression and control of the immune response. RFX5 is a protein consisting of 616 amino acids.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RFX5