RFC2 Antibody - BSA Free

Western Blot: RFC2 Antibody [NB100-231] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RFC2 antibody used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Western Blot: RFC2 Antibody [NB100-231] - Whole cell extract (50 ug) from MCF7 and 293T cells (A) or nuclear extract (50 ug) from HeLa cells (B). Antibody used at 0.5 ug/ml (A) and 1 ug/ml (B).

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

RFC2 Antibody - BSA Free Summary

• Description: Novus Biologicals Goat RFC2 Antibody - BSA Free (NB100-231) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residues 1 and 50 of human Replication Factor C subunit 2 using the numbering given in entry NP_852136.1 (GeneID 5982).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: RFC2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:5000
• Application Notes: This antibody can be used for Western Blot. *The investigator should determine the optimal dilution for a specific application.
• Control:

  RFC2 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RFC2 Antibody - BSA Free

• A1 40 kDa subunit
• A1
• Activator 1 40 kDa subunit
• Activator 1 subunit 2
• MGC3665
• replication factor C (activator 1) 2, 40kDa
• replication factor C 2
• Replication factor C 40 kDa subunit
• replication factor C subunit 2
• RF-C 40 kDa subunit
• RFC40replication factor C (activator 1) 2 (40kD)

Background

The Replication factor C (RFC) heteropentamer includes RFC1, RFC2, RFC3, RFC4, and RFC5. The RFC heteropentamer is part of the multiprotein DNA replicase that functions to replicate DNA prior to cell division and repair DNA damage. RFC is the clamp loader ATPase associated with the DNA replicase. As the clamp loader, RFC uses ATP to recruit, open, and close the PCNA ring to link it to DNA for replication and repair. RFC2 is the 40 kDa subunit of RFC that is part of the core complex formed with RFC4 and RFC5. RFC2 is also known as replication factor C subunit 2, replication factor C 40 kDa subunit, RF-C 40 kDa subunit, RFC40, activator 1 40 kDa subunit, A1 40 kDa subunit, A1, and MGC3665.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RFC2
• Entrez:
  • 5982(Human)
• Uniprot:
  • NP_852136.1(Human)