RFC1 Antibody

Images

 
Western Blot: RFC1 Antibody [NB100-230] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RFC1 ...read more
Biological Strategies: Western Blot: RFC1 Antibody [NB100-230] - Samples: Whole cell lysate (50, 15 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RFC1 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

RFC1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1100 and the C-terminus (residue 1148) of human Replication Factor C subunit 1 using the numbering given in entry NP_002904.3 (GeneID 5981).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
RFC1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoprecipitation 2-6 ug/mg extract
  • Western Blot 1:1000-1:10000
Application Notes
This antibody can be used for Western Blot and immunoprecipitation. Suggested working dilutions: * Western Blot 1:1,000-1:10,000 Immunoprecipitation 2-6 ug/mg extract Immunocytochemistry ND Immunohistochemistry ND *The investigator should determine the optimal dilution for a specific application.
Publications
Read Publication using
NB100-230 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RFC1 Antibody

  • A1 140 kDa subunit
  • A1
  • Activator 1 140 kDa subunit
  • Activator 1 large subunit
  • Activator 1 subunit 1
  • MGC51786
  • MHC binding factor, beta
  • MHCBFB
  • PO-GA
  • RECC1
  • replication factor C (activator 1) 1 (145kD)
  • replication factor C (activator 1) 1, 145kDa
  • Replication factor C 140 kDa subunit
  • Replication factor C large subunit
  • replication factor C subunit 1
  • replication factor C1
  • RF-C 140 kDa subunit
  • RFC
  • RFC1
  • RFC140
  • RFC140DNA-binding protein PO-GA

Background

The protein encoded by this gene is the large subunit of replication factor C, which is a five subunit DNA polymerase accessory protein. Replication factor C is a DNA-dependent ATPase that is required for eukaryotic DNA replication and repair. The protein acts as an activator of DNA polymerases, binds to the 3' end of primers, and promotes coordinated synthesis of both strands. It also may have a role in telomere stability. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RFC1 Antibody (NB100-230)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: ICC/IF.


Filter By Application
ICC/IF
(1)
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Filter By Species
Mouse
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional RFC1 Products

Array NB100-230

Research Areas for RFC1 Antibody (NB100-230)

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Blogs on RFC1

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Calculators

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Bioinformatics

Gene Symbol RFC1
Entrez
Uniprot