Rev-erb A alpha/NR1D1 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: Rev-erb A alpha/NR1D1 Antibody [NBP1-84931] - Staining of human cell line HaCaT shows localization to nuclear bodies. Antibody staining is shown in green.

• Reactivity: Hu, Am, Mu, Rt
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Rev-erb A alpha/NR1D1 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: TTLDSNNNTGGVITYIGSSGSSPSRTSPESLYSDNSNGSFQSLTQGCPTYFPPSPTGSLTQDPARSFGSIPPSLSDDGSPSSSSSSSSSSSSFYNGSPPGSLQVAMEDSSRVSPSKSTSNITKLNGMVLLCKVCGDVA
• Predicted Species: Mouse (96%), Rat (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NR1D1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry-Frozen Validated from a verified customer review.
• Application Notes: ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  Rev-erb A alpha/NR1D1 Protein (NBP1-84931PEP)

Reactivity Notes

Amphibian reactivity reported from a verified customer review.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Rev-erb A alpha/NR1D1 Antibody - BSA Free

• Ear-1
• EAR1nuclear receptor subfamily 1 group D member 1
• HREV
• NR1D1
• nuclear receptor subfamily 1, group D, member 1
• Reverb A alpha
• Rev-erb A alpha
• Rev-ErbAalpha
• Rev-erbA-alpha
• THRA1
• THRAL
• THRALrev-erbA-alpha
• V-erbA-related protein 1

Background

NR1D1 functions as a constitutive transcriptional repressor. Possible receptor for triiodothyronine

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Rev-erb A alpha/NR1D1 Antibody (NBP1-84931)(2)

Publications using NBP1-84931

• Zhu K, Celwyn IJ, Guan D et al. An intrinsically disordered region controlling condensation of a circadian clock component and rhythmic transcription in the liver Molecular cell 2023-10-05 [PMID: 37802023]
• Wang Q, Sundar IK, Lucas JH et al. Circadian clock molecule REV-ERB? regulates lung fibrotic progression through collagen stabilization Nature communications 2023-03-09 [PMID: 36894533] (Immunohistochemistry-Paraffin, Mouse)

Reviews for Rev-erb A alpha/NR1D1 Antibody (NBP1-84931) (2)

Average Rating: 4

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Human, hyla cinerea.

reviewed by: Deborah Lutterschmidt IHC-Fr hyla cinerea 10/23/2023

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Brain
• Species: hyla cinerea
• Lot: 000044560

Comments

• Comments: ***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.***

reviewed by: Liyong Zhang Immunocytochemistry/Immunofluorescence Human 12/15/2017

Summary

• Application: Immunocytochemistry/Immunofluorescence
• Sample Tested: human primary hepatocytes
• Species: Human
• Lot: A78474

Comments

• Comments: Name: Anti-NRIDI antibody (NBP1-84931)
  Catalog #: Anti-NRIDI antibody (NBP1-84931)
  Lot Number: Anti-NRIDI antibody (NBP1-84931, Lot # A78474)
  Fixation: 2% paraformaldehyde at room temperature for 15 min
  Permeability: 0.1% Triton X-100 in TBS for 5-10 minutes
  Blocking: 2% BSA in PBS for 1 hr at room temperature
  Primary antibody: iNOS, 1/200; NRIDI1/200, overnight at 4°C
  Secondary antibody: Goat anti-Rabbit IgG H&L at 1:500 in PBS+0.5% BSA (PBB)

Bioinformatics

• Gene Symbol: NR1D1