RelA/NFkB p65 Antibody (465003)

Daudi human Burkitt's lymphoma cell line was stained with Human RelA/NF kappa B p65 Monoclonal Antibody (Catalog # MAB50781, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol.

• Reactivity: Hu
• Applications: CyTOF-ready, Flow
• Clone: 465003
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

RelA/NFkB p65 Antibody (465003) Summary

• Immunogen: E. coli-derived recombinant human RelA/NF kappa B p65
  Met1-Thr254
  Accession # NP_068810
• Specificity: Detects human RelA/NF kappa B p65 in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: RELA
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for RelA/NFkB p65 Antibody (465003)

• MGC131774
• nf kb p65
• NFkB p65
• NF-kB p65
• NFKB3
• NFKB3v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor ofkappa light polypeptide gene enhancer in B-cells 3 (p65))
• Nuclear factor NF-kappa-B p65 subunit
• Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3transcription factor p65
• p65
• p65RelA
• rela p65
• RelA
• v-rel reticuloendotheliosis viral oncogene homolog A (avian)
• v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappalight polypeptide gene enhancer in B-cells 3, p65

Background

RelA p65 is a 65 kDa member of the NFkB family of nuclear transcription factors. Dimers of p65 with the p50 subunit are the most common form of the NFkB transcription factor, but dimers with itself or other family members are also active. An alternatively spliced isoform that lacks amino acids (aa) 222-231 (p65D) does not bind DNA. Over the sequence used as an immunogen, human RelA p65 shares 96% and 98% aa identity with mouse and rat RelA p65, respectively. This portion includes one of eight potential Ser/Thr phosphorylation sites, two acetylation sites, and most of the Rel homology domain that interacts with IkB inhibitors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RelA/NFkB p65 Antibody (MAB50781)(1)

Publication using MAB50781

• L Zhou, Y Huang, H Xie, X Mei Buyang Huanwu Tang improves denervation-dependent muscle atrophy by increasing ANGPTL4, and increases NF-?B and MURF1 levels Mol Med Rep, 2017-12-19;0(0):. 2017-12-19 [PMID: 29257347] (Western Blot, Rat)

Bioinformatics

• Gene Symbol: RELA
• Uniprot:
  • NP_068810()