RED1 Antibody

Product Discontinued

RED1 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: SVNTDFTSDQADFPDTLFNGFETPDKAEPPFYVGSNGDDSFSSSGDLSLSASPVPASLAQPPLPVLPPFPPPSGKNPVMILNELRPGLKYDFLSESGESHAKSFVMSVVV
• Predicted Species: Mouse (95%), Rat (95%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ADARB1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Simple Western 1:20
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
• Theoretical MW: 81 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23534823)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for RED1 Antibody

• ADAR2a
• ADAR2ADAR2g
• ADAR2a-L1
• ADAR2a-L2
• ADAR2a-L3
• ADAR2b
• ADAR2c
• adenosine deaminase, RNA-specific, B1
• double-stranded RNA-specific editase 1
• DRABA2
• DRADA2hRED1
• EC 3.5
• human dsRNA adenosine deaminase DRADA2
• human dsRNA adenosine deaminase DRADA2b, EC 3.510adenosine deaminase, RNA-specific, B1 (homolog of rat RED1)
• RED1 homolog
• RED1ADAR2d
• RNA editase
• RNA editing deaminase 1
• RNA-editing deaminase 1
• RNA-editing enzyme 1
• RNA-specific, B1 (RED1 homolog rat)

Background

RED1 encodes the enzyme responsible for pre-mRNA editing of the glutamate receptor subunit B by site-specific deamination of adenosines. Studies in rat found that this enzyme acted on its own pre-mRNA molecules to convert an AA dinucleotide to an AI dinucleotide which resulted in a new splice site. Alternative splicing of this gene results in several transcript variants, some of which have been characterized by the presence or absence of an ALU cassette insert and a short or long C-terminal region. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RED1 Antibody (NBP1-90240)(1)

Publication using NBP1-90240

• Witman NM, Behm M, Ohman M, Morrison JI. ADAR-related activation of adenosine-to-inosine RNA editing during regeneration. Stem Cells Dev 2013-08-15 [PMID: 23534823]

Bioinformatics

• Gene Symbol: ADARB1
• Entrez:
  • 104(Human)
• Uniprot:
  • P78563(Human)