Recombinant Rhesus Macaque IL-13 Protein, CF

Images

 
There are currently no images for IL-13 (2674-RM/CF).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity RMSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Recombinant Rhesus Macaque IL-13 Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 2-8 ng/mL.
Source
E. coli-derived rhesus macaque IL-13 protein
Ser19-Asn132
Accession #
N-terminal Sequence
Ser19
Protein/Peptide Type
Recombinant Proteins
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
12.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
2674-RM/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rhesus Macaque IL-13 Protein, CF

  • ALRHMGC116789
  • BHR1interleukin-13
  • IL13
  • IL-13
  • IL-13MGC116788
  • interleukin 13
  • MGC116786
  • NC30
  • P600

Background

IL-13 is a 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergic asthma and atopy. It is secreted by Th1 and Th2 CD4+ T cells, NK cells, visceral smooth muscle cells, eosinophils, mast cells, and basophils (1, 2). IL-13 circulates as a monomer with two internal disulfide bonds that contribute to a bundled four alpha -helix configuration (3, 4). Mature rhesus IL-13 shares 94%, 58%, and 60% amino acid sequence identity with human, mouse, and rat IL-13, respectively. Despite the low homology, it exhibits cross-species activity between human, mouse, and rat (5, 6). IL-13 has diverse activities on numerous cell types (7). On macrophages, IL-13 suppresses the production of proinflammatory cytokines and other cytotoxic substances. On B cells, IL-13 induces immunoglobulin class switching to IgE, upregulates the expression of MHC class II, CD71, CD72, and CD23, and costimulates proliferation. IL-13 upregulates IL-6 while downregulating IL-1 and TNF-alpha production by fibroblasts and endothelial cells. IL-13 binds with low affinity to IL-13 R alpha 1, triggering IL-13 R alpha 1 association with IL-4 R alpha. This high affinity receptor complex also functions as the type 2 IL-4 receptor complex (8, 9). Additionally, IL-13 binds with high affinity to IL-13 R alpha 2 which is expressed intracellularly, on the cell surface, and as a soluble molecule (10 - 13). IL-13 R alpha 2 regulates the bioavailability of both IL-13 and IL-4 and is overexpressed in glioma and several bronchial pathologies (9, 14, 15). Compared to wild type IL-13, the atopy-associated R110Q variant of IL-13 elicits increased responsiveness from eosinophils that express low levels of IL-13 R alpha 2 (16).

  1. Wills-Karp, M. (2004) Immunol. Rev. 202:175.
  2. Nakajima H. and K. Takatsu (2007) Int. Arch. Allergy Immunol. 142:265.
  3. Moy, F.J. et al. (2001) J. Mol. Biol. 310:219.
  4. Eisenmesser, E.Z. et al. (2001) J. Mol. Biol. 310:231.
  5. Ruetten, H. and C. Thiemermann (1997) Shock 8:409.
  6. Lakkis, F.G. et al. (1997) Biochem. Biophys. Res. Commun. 235:529. 
  7. Wynn, T.A. (2003) Annu. Rev. Immunol. 21:425.
  8. Andrews, A.L. et al. (2002) J. Biol. Chem. 277:46073.
  9. Tabata, Y. et al. (2007) Curr. Allergy Asthma Rep. 7:338.
  10. Chiaramonte, M.G. et al. (2003) J. Exp. Med. 197:687.
  11. Daines, M.O. and G.K. Hershey (2002) J. Biol. Chem. 227:10387.
  12. Matsumura, M. et al. (2007) Biochem. Biophys. Res. Commun. 360:464.
  13. Tabata, Y. et al. (2007) J. Immunol. 177:7905.
  14. Andrews, A.L. et al. (2006) J. Allergy Clin. Immunol. 118:858.
  15. Joshi, B.H. et al. (2006) Vitam. Horm. 74:479.
  16. Andrews, A.-L. et al. (2007) J. Allergy Clin. Immunol. 120:91.

Customers Who Viewed This Item Also Viewed...

NBP2-25241
Species: Hu, Mu
Applications: WB, ICC/IF, IP
NB600-922
Species: Ch, Vi
Applications: WB, ELISA, IHC, IHC-P, IP
NBP1-19371
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
AF152
Species: Hu
Applications: WB, Flow, IHC, CyTOF-ready, Neut
7954-GM/CF
Species: Hu
409-ML/CF
Species: Mu
NB7343
Species: Ca
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP1-53144
Species: Hu, Mu, Rt, Bv, Ca, Eq, GP, Rb, Ye, Ze
Applications: WB, IHC

Publications for IL-13 (2674-RM/CF)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Bioassay.


Filter By Application
Bioassay
(1)
All Applications
Filter By Species
Human
(1)
All Species

Reviews for IL-13 (2674-RM/CF) (0)

There are no reviews for IL-13 (2674-RM/CF). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for IL-13 (2674-RM/CF) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

Additional IL-13 Products

Diseases for IL-13 (2674-RM/CF)

Discover more about diseases related to IL-13 (2674-RM/CF).
 

Pathways for IL-13 (2674-RM/CF)

View related products by pathway.

PTMs for IL-13 (2674-RM/CF)

Learn more about PTMs related to IL-13 (2674-RM/CF).

Blogs on IL-13.

FLICE, FLICE, baby
Cell death via apoptosis is a fundamental cellular function triggered by the cell death receptor family and their ligands which signal through downstream adaptor molecules and the caspase protease family. All caspases exist in a precursor form compose...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Rhesus Macaque IL-13 Protein, CF and receive a gift card or discount.

Bioinformatics

Uniprot