Recombinant Rat Reg3G Protein, CF

• Reactivity: Rt
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Rat Reg3G Protein, CF Summary

• Details of Functionality: Measured in a cell proliferation assay using RT4‑D6P2T rat schwannoma cells. The ED₅₀ for this effect is 0.15-0.75 μg/mL.
• Source: E. coli-derived rat Reg3G protein
  Glu27-Ala174, with an N-terminal Met and a C-terminal 6-His tag
• Accession #: P42854
• N-terminal Sequence: Met
• Protein/Peptide Type: Recombinant Proteins
• Gene: Reg3g
• Purity: >95%, by SDS-PAGE with silver staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 17 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 17 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE with silver staining
• Reconstitution Instructions: Reconstitute at 500 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat Reg3G Protein, CF

• LPPM429
• MGC118999
• MGC119001
• Pancreatitis-associated protein 1B
• Pancreatitis-associated protein IB
• PAP IB
• PAP1B
• PAP-1B
• PAP1BMGC118998
• PAP3
• PAPIB
• PAPIII
• Reg III-gamma
• Reg3G
• REG-3-gamma
• regenerating gene III
• regenerating islet-derived 3 gamma
• regenerating islet-derived protein 3-gamma
• Regenerating islet-derived protein III-gamma
• REG-III
• UNQ429

Background

Regenerating islet-derived protein 3-gamma (Reg3G), also known as pancreatitis-associated protein 3 (PAP3), is one of several secreted Reg proteins that contain a single C-type lectin domain and contribute to anti-microbial immunity in the gut (1, 2). Reg3G is secreted as a 148 amino acid (aa) proprotein, and 11 residues are cleaved from the N-terminus to generate the 15 kDa mature protein (3). This short propeptide serves as an inhibitor that limits the activity of the proprotein (3, 4). Mature rat Reg3G shares 65% and 85% aa sequence identity with human and mouse Reg3G, respectively. Reg3G is expressed by Paneth cells of the small intestine and is released into the gut lumen (5). It is up-regulated in gastrointestinal epithelium by bacterial inflammation or by defects in the mucous lining (5-8). Its expression and secretion is also induced in the lung in response to MRSA (drug resistant S. aureus) and in damaged skeletal muscle and peripheral neurons (4, 9). Reg3G binds to bacterial peptidoglycans, exerts bacteriostatic and bactericidal activities, and protects the mucosa against bacterial invasion (4, 5, 10, 11). It also inhibits inflammation by limiting the number of Th1 cells in the lamina propria (11). R&D in-house data indicate that Reg3G can induce the proliferation of Schwann cells, a property shared with Reg3B (12).

1. Parikh, A. et al. (2012) Biomol. Concepts 3:57.
2. Narushima, Y. et al. (1997) Gene 185:159.
3. Mukherjee, S. et al. (2009) J. Biol. Chem. 284:4881.
4. Choi, S-M. et al. (2013) J. Exp. Med. 210:551.
5. Cash, H.L. et al. (2006) Science 313:1126.
6. Lee, K.S. et al. (2012) PLoS ONE 7:e30786.
7. Keilbaugh, S.A. et al. (2005) Gut 54:623.
8. Burger-van Passen, N. et al. (2012) PLoS ONE 7:e38798.
9. Klasan, G.S. et al. (2014) Muscle Nerve 49:61.
10. Brandl, K. et al. (2007) J. Exp. Med. 204:1891.
11. Vaishnava, S. et al. (2011) Science 334:255.
12. Livesey, F.J. et al. (1997) Nature 390:614.

Bioinformatics

• Gene Symbol: Reg3g
• Uniprot:
  • P42854()