Recombinant Rat PDGF-AA Protein, CF

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Product Details

Summary
Reactivity RtSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Rat PDGF-AA Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 3-9 ng/mL.
Source
E. coli-derived rat PDGF-AA protein
Ser87-Arg196
Accession #
N-terminal Sequence
Ser87
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Pdgfa
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
12.5 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
1055-AA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile 4 mM HCl.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat PDGF-AA Protein, CF

  • PDGF1
  • PDGF-A
  • PDGFAA
  • PDGF-AA

Background

The platelet-derived growth factor (PDGF) family consists of proteins derived from four genes (PDGF-A, -B, -C, and -D) that form four disulfide-linked homodimers (PDGF-AA, -BB, -CC, and -DD) and one heterodimer (PDGF-AB) (1, 2). These proteins regulate diverse cellular functions by binding to and inducing the homo- or hetero-dimerization of two receptors (PDGF R alpha and R beta ). Whereas alpha / alpha homo-dimerization is induced by PDGF-AA, -BB, -CC, and -AB, alpha / beta hetero-dimerization is induced by PDGF-AB,-BB, -CC, and -DD, and beta / beta homo-dimerization is induced only by PDGF-BB, and -DD (1 - 4). Both PDGF R alpha and R beta are members of the class III subfamily of receptor tyrosine kinases (RTK) that have five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. Ligand-induced receptor dimerization results in autophosphorylation in trans resulting in the activation of several intracellular signaling pathways that can lead to cell proliferation, cell survival, cytoskeletal rearrangement, cell migration and extracellular matrix production. Rat PDGF-A chain cDNA encodes a 204 amino acid (aa) residue precursor protein with a 20 aa signal peptide, a 65 aa propeptide that is removed by proteolysis, and a 119 aa mature protein. By alternative splicing, a short form lacking 8 C-terminal aa residues also exists. The long form contains the 8 aa basic insert which promotes intracellular cell retention and association with cell matrix. PDGF-A is expressed in multiple cell types and tissues. Based on PDGF-A knockout studies, PDGF-A appears to be important for the development of oligodendrocytes, testicular Leydig cells, alveolar smooth muscle cells, hair follicles and intestinal villus (1).

  1. Betshotz, C. et al. (2001) BioEssays 23:494.
  2. Ostman, A. and A.H. Heldin (2001) Advances in Cancer Research 80:1.
  3. Gilbertson, D. et al. (2001) J. Biol. Chem. 276:27406.
  4. LaRochells, W.J. et al. (2001) Nature Cell Biol. 3:517.

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Publications for PDGF-AA (1055-AA)(12)

We have publications tested in 4 confirmed species: Mouse, Rat, Chicken, Hamster.

We have publications tested in 4 applications: Bioassay, Cell Culture, In Vivo, Tissue Culture.


Filter By Application
Bioassay
(9)
Cell Culture
(1)
In Vivo
(1)
Tissue Culture
(1)
All Applications
Filter By Species
Mouse
(2)
Rat
(8)
Chicken
(1)
Hamster
(1)
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using 1055-AA Applications Species
T Shimizu, H Murakoshi, H Matsumoto, K Ichino, A Hattori, S Ueno, A Ishida, N Tajiri, H Hida Tension Sensor Based on Fluorescence Resonance Energy Transfer Reveals Fiber Diameter-Dependent Mechanical Factors During Myelination Frontiers in Cellular Neuroscience, 2021;15(0):685044. 2021 [PMID: 34408628] (Bioassay, Rat) Bioassay Rat
JL Kadrmas, MC Beckerle, M Yoshigi Genetic analyses in mouse fibroblast and melanoma cells demonstrate novel roles for PDGF-AB ligand and PDGF receptor alpha Sci Rep, 2020;10(1):19303. 2020 [PMID: 33168840] (Bioassay, Rat) Bioassay Rat
X Hao, X Guan, X Zhao, M Ji, X Wen, P Chen, F Chen, J Yang, Q Lian, L Ye, H Chen Phthalate inhibits Leydig cell differentiation and promotes adipocyte differentiation Chemosphere, 2020;262(0):127855. 2020 [PMID: 32799149] (Bioassay, Rat) Bioassay Rat
S Ogawa, M Hagiwara, S Misumi, N Tajiri, T Shimizu, A Ishida, N Suzumori, M Sugiura-Og, H Hida Transplanted Oligodendrocyte Progenitor Cells Survive in the Brain of a Rat Neonatal White Matter Injury Model but Less Mature in Comparison with the Normal Brain Cell Transplant, 2020;29(0):9.6369E+14. 2020 [PMID: 32757665] (Cell Culture, Rat) Cell Culture Rat
X Guan, P Chen, X Zhao, X Hao, F Chen, M Ji, X Wen, H Lin, L Ye, H Chen Characterization of stem cells associated with seminiferous tubule of adult rat testis for their potential to form Leydig cells Stem Cell Res, 2019;41(0):101593. 2019 [PMID: 31704538] (Tissue Culture, Rat) Tissue Culture Rat
Lopez-Bellido R, Puig S, Huang P, Tsai C, Turner H, Galko M, Gutstein H Growth Factor Signaling Regulates Mechanical Nociception in Flies and Vertebrates. J Neurosci, 0;39(30):6012-6030. 0 [PMID: 31138657] (In Vivo, Rat) In Vivo Rat
R Lopez-Bell, S Puig, PJ Huang, CR Tsai, HN Turner, MJ Galko, HB Gutstein Growth factor signaling regulates mechanical nociception in flies and vertebrates J. Neurosci., 2019;0(0):. 2019 [PMID: 31138657] (Bioassay, Rat) Bioassay Rat
Retinal Proteoglycans Act as Cellular Receptors for Basement Membrane Assembly to Control Astrocyte Migration and Angiogenesis Cell Rep, 2016;17(7):1832-1844. 2016 [PMID: 27829154] (Bioassay, Mouse) Bioassay Mouse
Poehler A, Xiang W, Spitzer P, May V, Meixner H, Rockenstein E, Chutna O, Outeiro T, Winkler J, Masliah E, Klucken J Autophagy modulates SNCA/alpha-synuclein release, thereby generating a hostile microenvironment. Autophagy, 2014;10(12):2171-92. 2014 [PMID: 25484190] (Bioassay, Rat) Bioassay Rat
Yang J, Wang J, Pan L, Li H, Rao C, Zhang X, Niu G, Qu J, Hou L BMP4 is required for the initial expression of MITF in melanocyte precursor differentiation from embryonic stem cells. Exp Cell Res, 2014;320(1):54-61. 2014 [PMID: 24080013] (Bioassay, Mouse) Bioassay Mouse
Show All 12 Publications.

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Bioinformatics

Gene Symbol Pdgfa
Uniprot