Recombinant Rat Neuroligin 1/NLGN1 Protein, CF

• Reactivity: Rt
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Rat Neuroligin 1/NLGN1 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When recombinant rat Neuroligin 1 is immobilized at 5 μg/mL, biotinylated recombinant human Neurexin 1 beta Fc Chimera binds with an apparent K_D <20 nM.
• Source: Mouse myeloma cell line, NS0-derived rat Neuroligin 1/NLGN1 protein
  Met1-Leu676, with a C-terminal 6-His tag
• Accession #: Q62765 (isoform 4)
• N-terminal Sequence: No results obtained: Gln46 predicted
• Protein/Peptide Type: Recombinant Proteins
• Gene: Nlgn1
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 71 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 85-105 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat Neuroligin 1/NLGN1 Protein, CF

• EC 3.1.1
• EC 3.1.1.1
• KIAA1070neuroligin-1
• MGC45115
• Neuroligin 1
• NL1
• Nlg
• NLGN1

Background

Neuroligin 1 (NLGN1 or NL1) is one of several type I transmembrane Neuroligin proteins that play an important role in synaptic development (1). Mature rat Neuroligin 1 is an N- and O-glycosylated 120 kDa molecule that consists of a 652 amino acid (aa) extracellular domain (ECD) with a catalytically inactive cholinesterase-like domain, a 21 aa transmembrane segment, and a 125 aa cytoplasmic tail (2). Insertion of 20 aa at splice site A and/or 9 aa at splice site B within the ECD results in NLGN1A, NLGN1B, or NLGN1AB isoforms while the NLGN1(-) isoform has neither insertion (3). This recombinant protein (NLGN1B) contains the insert at splice site B but not the insert at splice site A. Within the ECD, rat NLGN1B shares 99% aa sequence identity with comparable regions of human and mouse Neuroligin 1. Neuroligin 1 expression is restricted to postsynaptic nerve terminal membranes where it associates into dimers and tetramers (5 - 7). NLGN1B and NLGN1AB are enriched at excitatory (glutamatergic) contacts, whereas NLGN1A is enriched at inhibitory (GABAergic) contacts and NLGN1(-) is expressed at both (3). The interaction of Neuroligin 1 with presynaptic membrane Neurexins promotes the maturation of presynaptic as well as postsynaptic structures (3, 4, 8 - 11). Neuroligin 1 isoforms are selective in which Neurexin isoforms they bind: NLGN1B and NLGN1AB preferentially interact with Neurexin 1 beta lacking the splice site 4 insert, while NLGN1A and NLGN1(-) bind Neurexin 1 alpha and Neurexin 1 beta irrespective of the SS4 insert (12). Neurexin 1 beta (-SS4) additionally binds to LRRTM2 to trigger a second mechanism that promotes excitatory synapse development (13, 14). Neuroligin 1 mediated synaptogenesis is also enhanced by its direct binding to Thrombospondin 1 (15).

1. Sudhof, T.C. (2008) Nature 455:903.
2. Ichtchenko, K. et al. (1995) Cell 81:435.
3. Chih, B. et al. (2006) Neuron 51:171.
4. Dean, C. et al. (2003) Nat. Neurosci. 7:708.
5. Comoletti, D. et al. (2006) Biochemistry 45:12816.
6. Berninghausen, O. et al. (2007) J. Neurochem. 103:1855.
7. Song, J.-Y. et al. (1999) Proc. Natl. Acad. Sci. 96:1100.
8. Wittenmayer, N. et al. (2009) Proc. Natl. Acad. Sci. 106:13564.
9. Jung, S.-Y. et al. (2010) Proc. Natl. Acad. Sci. 107:4710.
10. Chubykin, A.A. et al. (2007) Neuron 54:919.
11. Chih, B. et al. (2005) Science 307:1324.
12. Boucard, A.A. et al. (2005) Neuron 48:229.
13. Siddiqui, T.J. et al. (2010) J. Neurosci. 30:7495.
14. Ko, J. et al. (2009) Neuron 64:791.
15. Xu, J. et al. (2010) Nat. Neurosci. 13:22.

Publications for Neuroligin 1/NLGN1 (4340-NL)(2)

Publications using 4340-NL

• KDB Wierda, TL Toft-Berte, CR Gøtzsche, E Pedersen, I Korshunova, J Nielsen, ML Bang, AB Kønig, S Owczarek, MD Gjørlund, M Schupp, E Bock, JB Sørensen The soluble neurexin-1&amp;beta ectodomain causes calcium influx and augments dendritic outgrowth and synaptic transmission Sci Rep, 2020-10-22;10(1):18041. 2020-10-22 [PMID: 33093500] (Surface Plasmon Resonance, Rat)
• J Brito-More, MV Lourenco, MM Oliveira, JF Vital, FC Ribeiro, JH Ledo, LP Diniz, MH Magdesian, HM Melo, F Barros-Ara, JM de Souza, SV Alves-Leon, FC Gomes, JR Clarke, CP Figueiredo, FG De Felice, ST Ferreira Interaction of A? Oligomers with Neurexin 2? and Neuroligin 1 Mediates Synapse Damage and Memory Loss in Mice J. Biol. Chem, 2017-03-10;0(0):. 2017-03-10 [PMID: 28283575] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Nlgn1
• Uniprot:
  • Q62765 (isoform 4)()