Recombinant Rat FGF-BP Protein

• Reactivity: Rt
• Applications: Binding Activity

Recombinant Rat FGF-BP Protein Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. Immobilized rhFGF-basic at 5 µg/mL (100 µL/well) can bind rrFGF-BP with a linear range of 0.8-50 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived rat FGF-BP protein Glu21-Cys238, with a C-terminal Asp and 10-His tag
• Accession #: Q9QY10
• N-terminal Sequence: Glu21
• Protein/Peptide Type: Recombinant Proteins
• Gene: Fgfbp1
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 26 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: Multiple bands between 25-37 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat FGF-BP Protein

• 17 kDa heparin-binding growth factor-binding protein
• FGFBP
• FGF-BP
• FGFBP1
• FGF-BP1
• FGFBP-1
• FGFBPFGF-binding protein 1
• fibroblast growth factor binding protein 1
• fibroblast growth factor-binding protein 1
• HBp17
• HBP1717 kDa HBGF-binding protein
• heparin-binding growth factor binding protein

Background

Fibroblast growth factor binding protein (FGF-BP), also known as HBp17, is a secreted glycoprotein that increases the bioavailability of FGFs (1). Mature FGF-BP is a 34 kDa O-glycosylated protein with five conserved intrachain disulfide bonds (2 - 4). FGF-BP contains a heparin-binding domain (aa 113 - 146) and a distinct FGF-binding region (aa 197 - 238) (5). Mature rat FGF-BP shares 54% and 81% aa sequence identity with mouse and rat FGF-BP, respectively. FGF-BP is expressed throughout development and in adult squamous epithelium (6, 7). It is upregulated in injured skin, renal tubular epithelium, and spinal nerves as well as in carcinomas of the skin, colon, and pancreas (3, 8 - 11). FGF-BP binds FGF -1, -2, -7, -10, and -22 which are secreted and sequestered in the extracellular matrix (ECM) (8, 12). The interactions of FGF-BP with heparin sulfate proteoglycans (HSPG) and FGF, modulates their activities (7, 9 13, 14). FGF-BP enhances the mitogenic effects of FGFs, thereby contributing to epithelial, endothelial, and neuronal tissue repair, angiogenesis, and tumor growth (8 - 10, 12, 15, 16).

1. Abuharbeid, S. et al. (2006) Int. J. Biochem. Cell Biol. 38:1463.
2. Aigner, A. et al. (2000) Growth Factors 18:51.
3. Tassi, E. et al. (2006) Cancer Res. 66:1191.
4. Lametsch, R. et al. (2000) J. Biol. Chem. 275:19469.
5. Xie, B. et al. (2006) J. Biol. Chem. 281:1137.
6. Aigner, A. et al. (2002) Histochem. Cell Biol. 117:1.
7. Wu, D. et al. (1991) J. Biol. Chem. 266:16778.
8. Beer, H.-D. et al. (2005) Oncogene 24:5269.
9. Ray, P.E. et al. (2006) Am. J. Physiol. Regul. Integr. Comp. Physiol. 290:R105.
10. Tassi, E. et al. (2007) Am. J. Physiol. Regul. Integr. Comp. Physiol. 293:R775.
11. Kurtz, A. et al. (2004) Neoplasia 6:595.
12. Tassi, E. et al. (2001) J. Biol. Chem. 276:40247.
13. Mongiat, M. et al. (2001) J. Biol. Chem. 276:10263.
14. Kurtz, A. et al. (1997) Oncogene 14:2671.
15. Aigner, A. et al. (2001) Int. J. Cancer 92:510.
16. Czubayko, F. et al. (1997) Nat. Med. 3:1137.

Publications for FGF-BP (1413-FB)(1)

Publication using 1413-FB

• Beer HD, Bittner M, Niklaus G, Munding C, Max N, Goppelt A, Werner S The fibroblast growth factor binding protein is a novel interaction partner of FGF-7, FGF-10 and FGF-22 and regulates FGF activity: implications for epithelial repair. Oncogene, 2005-08-11;24(34):5269-77. 2005-08-11 [PMID: 15806171] (Bioassay, Rat)

Bioinformatics

• Gene Symbol: Fgfbp1
• Uniprot:
  • Q9QY10()