Recombinant Rat CCL27/CTACK Protein, CF

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Product Details

Summary
Reactivity RtSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Rat CCL27/CTACK Protein, CF Summary

Details of Functionality
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR10. The ED50 for this effect is 0.15-0.75 µg/mL.
Source
E. coli-derived rat CCL27/CTACK protein
Leu70-Lys164
Accession #
N-terminal Sequence
Leu70
Protein/Peptide Type
Recombinant Proteins
Gene
Ccl27
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
10.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat CCL27/CTACK Protein, CF

  • ALP
  • C-C motif chemokine 27
  • CCL27
  • chemokine (C-C motif) ligand 27
  • CTACK
  • CTACKSmall-inducible cytokine A27
  • CTAK
  • cutaneous T-cell attracting chemokine
  • Cutaneous T-cell-attracting chemokine
  • ESKINE
  • IL-11 Ralpha-locus chemokine
  • ILC
  • ILCCC chemokine ILC
  • PESKY
  • SCYA27IL-11 R-alpha-locus chemokine
  • skinkine
  • small inducible cytokine subfamily A (Cys-Cys), member 27

Background

CCL27, also known as CTACK (cutaneous T cell-attracting chemokine), ALP, ILC, and ESkine, is a member of the CC family of chemokines (1). Sequence comparison of the rat CCL27 with the mature human and mouse CCL27 suggests that the mature rat CCL27 is a 95 amino acid (aa) protein. It shares 57% and 87% aa sequence identity with human and mouse CCL27, respectively (2). It shares 16% - 33% aa sequence identity with other rat CC chemokines. An alternately spliced form of mouse CCL27, known as PESKY, is localized to the nucleus and promotes cellular migration (3). CCL27 is constitutively expressed by keratinocytes and is upregulated by inflammatory stimuli and wounds in skin (4 - 7). CCL27 binds CCR10, glycosaminoglycans in the extracellular matrix, sulfated tyrosine residues on PSGL-1, and determinants on the surface of fibroblasts and endothelial cells (5, 7 - 9). CCL27 cooperates with CCL17/TARC in inducing the migration of cutaneous lymphocyte antigen (CLA) positive memory T cells to the skin during inflammation (4, 6, 10 - 12). Endothelial cell-bound CCL27 can mediate the adhesion of endothelium to CLA+ T cells (6). CCL27 also induces the migration of keratinocyte precursors from bone marrow to the skin, thereby promoting wound healing (7). In humans, serum CCL27 levels are elevated and correlate with disease severity in atopic dermatitis, psoriasis vulgaris, and mycosis fungoides (13 - 15).

  1. Kunkel, L. and E.C. Butcher (2002) Immunity 16:1. 
  2. Accession # NP_001102130. 
  3. Gortz, A. et al. (2002) J. Immunol. 169:1387. 
  4. Morales, J. et al. (1999) Proc. Natl. Acad. Sci. 96:14470. 
  5. Homey, B. et al. (2000) J. Immunol. 164:3465. 
  6. Homey, B. et al. (2002) Nat. Med. 8:157. 
  7. Inokuma, D. et al. (2006) Stem Cells 24:2810. 
  8. Jarmin, D. et al. (2000) J. Immunol. 164:3460.
  9. Hirata, T. et al. (2004) J. Biol. Chem. 279:51775.
  10. Vestergaard, C. et al. (2004) Exp. Dermatol. 13:551.
  11. Reiss, Y. et al. (2001) J. Exp. Med. 194:1541.
  12. Soler, D. et al. (2003) Blood 101:1677.
  13. Kakinuma, T. et al. (2003) J. Allergy Clin. Immunol. 111:592.
  14. Hijnen, D. et al. (2004) J. Allergy Clin. Immunol. 113:334.
  15. Fujita, Y. et al. (2006) Clin. Cancer Res. 12:2670.

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Bioinformatics

Gene Symbol Ccl27
Uniprot