Recombinant Mouse XCL1/Lymphotactin Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse XCL1/Lymphotactin Protein, CF Summary

• Details of Functionality: Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human XCR1. The ED₅₀ for this effect is 0.05‑0.1 µg/mL.
• Source: E. coli-derived mouse XCL1/Lymphotactin protein
  Val22-Gly114, with an N-terminal Met
• Accession #: AAA56752.1
• N-terminal Sequence: Met
• Protein/Peptide Type: Recombinant Proteins
• Gene: Xcl1
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 10 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse XCL1/Lymphotactin Protein, CF

• ATAC
• ATACSmall-inducible cytokine C1
• C motif chemokine 1
• chemokine (C motif) ligand 1
• Cytokine SCM-1
• LPTNSCM-1-alpha
• LTNXC chemokine ligand 1
• Lymphotactin
• lymphotaxin
• SCM-1 alpha
• SCM-1
• SCM1A
• SCM-1a
• SCYC1SCM1
• single cysteine motif 1a
• small inducible cytokine subfamily C, member 1 (lymphotactin)
• XCL1

Background

Mouse lymphotactin (Lptn) and its human homologue (also named human SCM-1 and ATAC) belong to the C or gamma subfamily of chemokines. The C chemokines lack two (the 1st and 3rd) of the four invariant cysteine residues normally found in the CC and CXC chemokines and have an extended carboxy terminus. Mouse lymphotactin encodes a 114 amino acid residue precursor protein with a 21 amino acid residue predicted signal peptide. The expression of lymphotactin is restricted to activated mouse pro-T cells and to activated, class I MHC restricted T cells. The gene for lymphotactin has been mapped to chromosome 1 in both human and mouse. Recombinant mouse lymphotactin has been shown to have chemotactic activity for lymphocytes, NK cells and mouse splenocytes.

1. Kelner, G. et al. (1994) Science 266:1395.
2. Hedrick, J.A. et al. (1997) J. Immunol. 158:1533.

Publications for XCL1/Lymphotactin (486-LT/CF)(8)

Publications using 486-LT/CF

• Y Mizumoto, H Hemmi, M Katsuda, M Miyazawa, Y Kitahata, A Miyamoto, M Nakamori, T Ojima, K Matsuda, M Nakamura, K Hayata, Y Fukuda-Oht, M Sugiyama, T Ohta, T Orimo, S Okura, I Sasaki, K Tamada, H Yamaue, T Kaisho Anticancer effects of chemokine-directed antigen delivery to a cross-presenting dendritic cell subset with immune checkpoint blockade Br. J. Cancer, 2020-02-18;0(0):. 2020-02-18 [PMID: 32066911] (Cell Culture, Mouse)
• S Sharif, N Ueda, Y Nakatani, LM Wise, S Clifton, Z Lateef, AA Mercer, SB Fleming Chemokine-Binding Proteins Encoded by Parapoxvirus of Red Deer of New Zealand Display Evidence of Gene Duplication and Divergence of Ligand Specificity Front Microbiol, 2019-06-25;10(0):1421. 2019-06-25 [PMID: 31293551] (Binding Assay, Mouse)
• JP Böttcher, E Bonavita, P Chakravart, H Blees, M Cabeza-Cab, S Sammicheli, NC Rogers, E Sahai, S Zelenay, C Reis E Sou NK Cells Stimulate Recruitment of cDC1 into the Tumor Microenvironment Promoting Cancer Immune Control Cell, 2018-02-08;172(5):1022-1037.e14. 2018-02-08 [PMID: 29429633] (Bioassay, Mouse)
• K Li, G Wei, Y Cao, D Li, P Li, J Zhang, H Bao, Y Chen, Y Fu, P Sun, X Bai, X Ma, Z Lu, Z Liu The Identification and Distribution of Cattle XCR1 and XCL1 among Peripheral Blood Cells: New Insights into the Design of Dendritic Cells Targeted Veterinary Vaccine PLoS ONE, 2017-01-27;12(1):e0170575. 2017-01-27 [PMID: 28129380] (Bioassay, Bovine)
• Myster F, Palmeira L, Sorel O, Bouillenne F, DePauw E, Schwartz-Cornil I, Vanderplasschen A, Dewals B Viral semaphorin inhibits dendritic cell phagocytosis and migration but is not essential for gammaherpesvirus-induced lymphoproliferation in malignant catarrhal fever. J Virol, 2015-01-14;89(7):3630-47. 2015-01-14 [PMID: 25589653] (Bioassay, Ovine)
• Yamanaka H, Hoyt T, Yang X, Bowen R, Golden S, Crist K, Becker T, Maddaloni M, Pascual DW A parenteral DNA vaccine protects against pneumonic plague. Vaccine, 2010-03-01;28(18):3219-30. 2010-03-01 [PMID: 20197132] (ELISA (Standard))
• Rogers CJ, Zaharoff DA, Hance KW, Perkins SN, Hursting SD, Schlom J, Greiner JW Exercise enhances vaccine-induced antigen-specific T cell responses. Vaccine, 2008-08-14;26(42):5407-15. 2008-08-14 [PMID: 18706954] (In Vivo, Mouse)
• Ordway D, Higgins DM, Sanchez-Campillo J, Spencer JS, Henao-Tamayo M, Harton M, Orme IM, Gonzalez Juarrero M XCL1 (lymphotactin) chemokine produced by activated CD8 T cells during the chronic stage of infection with Mycobacterium tuberculosis negatively affects production of IFN-gamma by CD4 T cells and participates in granuloma stability. J. Leukoc. Biol., 2007-08-15;82(5):1221-9. 2007-08-15 [PMID: 17699612] (In Vivo, Mouse)

Bioinformatics

• Gene Symbol: Xcl1
• Uniprot:
  • AAA56752.1()