Recombinant Mouse TREML1/TLT-1 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse TREML1/TLT-1 Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of thrombin-activated human platelets.

  When 5 x 10⁶ cells per well are added to rmTLT-1 coated plates (2 μg/mL, 100 μL/well), approximately 50%-70% will adhere after 1 hour at 37 °C.

  Optimal dilutions should be determined by each laboratory for each application.

• Source: Mouse myeloma cell line, NS0-derived mouse TREML1/TLT-1 protein
  Gly18-Cys178 with a C-terminal 6-His tag
• Accession #: AAL85488
• N-terminal Sequence: Gly18
• Protein/Peptide Type: Recombinant Proteins
• Gene: Treml1
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 18.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 21-23 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse TREML1/TLT-1 Protein, CF

• dJ238O23.3
• GLTL1825
• PRO3438
• TLT1
• TLT-1
• TLT1MGC119173
• TREML1
• trem-like transcript 1 protein
• triggering receptor expressed on myeloid cells-like 1
• Triggering receptor expressed on myeloid cells-like protein 1

Background

Triggering receptor expressed on myeloid cells-like protein 1 (TREML1)/TREM-like transcript-1 (TLT-1) is a 40 kDa type I transmembrane receptor and member of the TREM family of proteins (1). Mouse TREML1/TLT-1 is synthesized as a 317 amino acid (aa) precursor that contains a 20 aa signal sequence, a 155 aa extracellular domain (ECD), a 21 aa transmembrane region, and a 121 aa cytoplasmic domain (SwissProt #: Q8K558). The ECD contains an Ig-like V-type domain (aa 21 - 122), and the cytoplasmic region contains a proline-rich region (aa 245 - 275) and an immunoreceptor tyrosine-based inhibition motif (ITIM) characterized by the residues S/I/V/LXYXXV/L (1). A splicing variant produces a second isoform that has a six aa substitution for aa 173 in isoform 1. Mature mouse TREML1/TLT-1 shares 65% aa sequence identity with mature human TREML1/TLT-1. TREML1/TLT-1 is expressed exclusively in megakaryocytes and platelets where it colocalizes with CD62P in alpha -granules in resting platelets and on the cell surface of platelets activated by thrombin (1 - 3). The receptor’s expression is upregulated dramatically upon platelet activation (2). Antibodies to single-chain Fv fragments specific for TLT-1 inhibited thrombin-mediated human platelet aggregation suggesting its role in the regulation of aggregation (5). In addition, soluble fragments including the ECD can be released into serum by proteolysis (3). The phosphorylated TREML1/TLT-1 is able to interact with both SHP-1 and SHP-2 through ITIM (1, 4). SHP-2 interaction enhances Fc epsilon RI-mediated calcium signaling in rat basophilic leukemia cells (1). It is hypothesized that TREML1/TLT-1 plays a role in the innate and adaptive immune responses.

1. Barrow, A.D. et al. (2004) J. Immunol. 172:5838.
2. Washington, A.V. et al. (2004) Blood 104:1042.
3. Gattis, J.L. et al. (2006) J. Biol. Chem. 281:13396.
4. Washington, A.V. et al. (2002) Blood 100:3822.
5. Giomarelli B. et al. (2007) Thromb. Haemost. 97:955.

Bioinformatics

• Gene Symbol: Treml1
• Uniprot:
  • AAL85488()