Recombinant Mouse TINAGL1 Protein, CF

Recombinant Mouse TINAGL1 (Catalog #9208-TI) binds to Cultrex Mouse Laminin I in an ELISA Binding Assay. When Cultrex Mouse Laminin I (Catalog # 3400-010-01) is coated at 5 µg/mL, Recombinant Mouse TINAGL1 binds with an ED50 = 1-6 ng/mL.

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse TINAGL1 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Cultrex Mouse Laminin I (Catalog # 3400-010-01) is coated at 5 µg/mL, Recombinant Mouse TINAGL1 binds with an
  ED₅₀ = 1-6 ng/mL.
• Source: Human embryonic kidney cell, HEK293-derived mouse TINAGL1 protein
  Asp41-His466, with a C-terminal 6-His tag
• Accession #: Q99JR5
• N-terminal Sequence: Asp41
• Protein/Peptide Type: Recombinant Enzymes
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 49 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 49-58 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
• Buffer: Supplied as a 0.2 μm filtered solution in Tris and NaCl.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse TINAGL1 Protein, CF

• androgen-regulated gene 1
• ARG1
• GIS5
• Glucocorticoid-inducible protein 5
• LCN7
• LIECG3
• lipocalin 7
• OLRG2
• OLRG-2
• Oxidized LDL-responsive gene 2 protein
• oxidized-LDL responsive gene 2
• P3ECSL
• TIN Ag-related protein
• TINAGL
• TINAGL1
• TINAG-like 1
• TINAGRP
• TIN-ag-RP
• tubulointerstitial nephritis antigen-like 1
• tubulointerstitial nephritis antigen-like
• Tubulointerstitial nephritis antigen-related protein

Background

Tubulointerstitial Nephritis Antigen-like (TINAGL1), also known as Lipocalin-7, AZ-1, and Arg1, is an approximately 50 kDa matricellular protein that contains a Somatomedin-B like domain, a vWF-C domain, and a cysteine peptidase domain that lacks the critical active site Cys residue (1, 2). Mouse TINAGL1 shares 90% and 97% aa sequence identity with human and rat TINAGL1, respectively. It is a component of the extracellular matrix in basement membranes (3-5) where it binds to Collagens I and IV, Laminin, Integrins a5 and b1, and the Anastellin fragment of Fibronectin (3-5). TINAGL1 promotes cell adhesion and angiogenic sprouting from vascular endothelial cells (3, 6). It is expressed in the adrenal cortex and medulla, vascular smooth muscle cells, cardiac and skeletal muscle cells, and in blastocysts that are competent for implantation (2, 3, 5, 7).

1. Kobayashi, M. et al. (2001) J. Steroid Biochem. Mol. Biol. 77:109.
2. Wex, T. et al. (2001) Biochemistry 40:1350.
3. Li, D. et al. (2007) FEBS Lett. 274:2506.
4. Tajiri, Y. et al. (2010) Biol. Reprod. 82:263.
5. Igarashi, T. et al. (2009) Biol. Reprod. 81:948.
6. Brown, L.J. et al. (2010) PLoS One 5:e13905.
7. Mukai, K. et al. (2003) J. Biol. Chem. 278:17084.

Bioinformatics

• Uniprot:
  • Q99JR5()