Recombinant Mouse TIM-2 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse TIM-2 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA.

  Immobilized Recombinant Mouse TIM-2 (0.5 μg/mL, 100 μL/well) can bind H-ferritin with an apparent K_D <3 nM.

  Optimal dilutions should be determined by each laboratory for each application.

• Source: Mouse myeloma cell line, NS0-derived mouse TIM-2 protein
  Met1-Asn229, with a C-terminal 6-His tag
• Accession #: NP_599010
• N-terminal Sequence: His22
• Protein/Peptide Type: Recombinant Proteins
• Gene: Timd2
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 23.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 40-60 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse TIM-2 Protein, CF

• G9D3
• T-cell immunoglobulin and mucin domain-containing protein 2
• T-cell immunoglobulin mucin receptor 2
• T-cell membrane protein 2
• TIM2
• TIM-2
• TIMD-2

Background

T cell immunoglobulin and mucin domain‑2 (TIM‑2) is an approximately 50 kDa member of the TIM family of immune regulatory proteins. TIMs are type I transmembrane proteins with one Ig‑like V domain and a Ser/Thr‑rich mucin domain in their extracellular regions (1). Mature mouse TIM‑2 consists of a 210 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 53 aa cytoplasmic domain (2). Rat TIM‑2, by contrast, possesses the same structural architecture as mouse but contains an additional 46 aa within the mucin domain. Over comparable regions of the ECD, mouse and rat TIM‑2 share 74% aa sequence identity. The isolated ECD forms dimers and tetramers in solution, and these structures suggest the possibility of TIM‑2/TIM‑2 cis interactions (3). TIM‑2 is expressed on Th2 cells, B cells, oligodendrocytes, hepatocytes, hepatic stellate cells, and epithelial cells lining the bile duct and renal tubules (4 ‑ 9). It is upregulated on Th2 cells during cellular activation and functions as a negative regulator of Th2 cell expansion and immune responses (8 ‑ 11). TIM‑2 binds to ligands expressed on activated B cells, macrophages, dendritic cells, and T cells (8, 12). Its ligation by Semaphorin 4A induces tyrosine phosphorylation in the cytoplasmic domain of TIM‑2 (12). TIM‑2 binding to ligand(s) on hepatocytes inhibits hepatocyte differentiation and proliferation (7). TIM‑2 additionally plays a role in iron homeostasis by serving as an endocytic receptor for the heavy chain of Ferritin (H‑Ferritin) (4, 6).

1. Rodriguez-Manzanet, R. et al. (2009) Immunol. Rev. 229:259.
2. McIntire, J.J. et al. (2001) Nat. Immunol. 2:1109.
3. Santiago, C. et al. (2007) Immunity 26:299.
4. Todorich, B. et al. (2008) J. Neurochem. 107:1495.
5. Ruddell, R.G. et al. (2009) Hepatology 49:887.
6. Chen, T.T. et al. (2005) J. Exp. Med. 202:955.
7. Watanabe, N. et al. (2007) Hepatology 45:1240.
8. Chakravarti, S. et al. (2005) J. Exp. Med. 202:437.
9. Rennert, P.D. et al. (2006) J. Immunol. 177:4311.
10. Fukushima, A. et al. (2007) Immunol. Lett. 110:133.
11. Knickelbein, J.E. et al. (2006) J. Immunol. 177:4966.
12. Kumanogoh, A. et al. (2002) Nature 419:629.

Publications for TIM-2 (1885-TM)(1)

Publication using 1885-TM

• A novel affinity-based method for the isolation of highly purified extracellular vesicles Sci Rep, 2016-09-23;6(0):33935. 2016-09-23 [PMID: 27659060] (Bioassay, Human)

Bioinformatics

• Gene Symbol: Timd2
• Uniprot:
  • NP_599010()