Recombinant Mouse Thrombospondin-4 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Thrombospondin-4 Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of SVEC4‑10 mouse vascular endothelial cells. The ED₅₀ for this effect is 0.1-0.5 μg/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived mouse Thrombospondin-4 protein
  Gln27-Asn963, with a C-terminal 6-His tag
• Accession #: Q9Z1T2
• N-terminal Sequence: Gln27 predicted: No results obtained, sequencing might be blocked
• Protein/Peptide Type: Recombinant Proteins
• Gene: Thbs4
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 104.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 120-145 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Thrombospondin-4 Protein, CF

• THBS4
• thrombospondin 4
• Thrombospondin4
• Thrombospondin-4
• TSP4
• TSP-4

Background

Thrombospondin-4 (TSP-4) is an approximately 140 kDa matricellular protein that is secreted as a disulfide-linked pentamer. Within the Thrombospondin family, TSP‑3 and TSP‑5/COMP are also pentameric, while TSP‑1 and TSP‑2 are trimeric. TSP‑4 regulates cell‑cell and cell matrix interactions and plays a role in cardiovascular physiology and neuronal development (1, 2). Mature mouse TSP‑4 consists of an N‑terminal heparin-binding domain, a coiled coil motif, four EGF‑like repeats, seven TSP type‑3 repeats (one with an RGD motif), and a TSP C‑terminal domain (2, 3). Mouse TSP‑4 shares 93% and 97% amino acid sequence identity with human and rat TSP‑4, respectively. TSP‑4 binds a variety of matrix proteins including Collagens I, II, III, V, Laminin‑1, Fibronectin, and Matrilin‑2 (4). Interactions of TSP‑4 with non‑collagenous proteins are independent of divalent cations, whereas interactions with collagenous proteins are enhanced in the presence of zinc (4). TSP‑4 binds to cell surface Integrins containing the alpha M, beta 2, or beta 3 chains (5, 6). It is expressed in skeletal muscle and tendon as well as by vascular smooth muscle and endothelial cells (7‑9). It is up‑regulated in cardiomyocytes during pressure overload and is required for mediating the responsive increase in cardiac contractility (10). In humans, a polymorphism of TSP-4 (A387P) is associated with myocardial infarction (11). TSP-4 contributes to the development of inflammation and atherosclerosis by promoting macrophage and neutrophil adhesion to the vasculature (5, 6). In the nervous system, TSP‑4 is expressed by astrocytes and neurons and is enriched at neuromuscular junctions and synapse‑rich layers of the brain and retina (9, 12, 13). It promotes neuronal adhesion, neurite outgrowth, and excitatory synaptogenesis (9, 13, 14). TSP‑4 is up‑regulated in the spinal cord following peripheral nerve injury where it contributes to presynaptic hypersensitivity and hyperalgesia (12). It is also up‑regulated in muscle following denervation (9). TSP‑4 is additionally secreted by tumor‑associated fibroblasts in gastric adenocarcinoma, particularly in regions of tumor cell invasion (15).

1. Murphy-Ullrich, J.E. and R.V. Iozzo (2012) Matrix Biol. 31:152.
2. Lawler, J. et al. (1995) J. Biol. Chem. 270:2809.
3. Newton, G. et al. (1999) Mamm. Genome 10:1010.
4. Narouz-Ott, L. et al. (2000) J. Biol. Chem. 275:37110.
5. Pluskota, E. et al. (2005) Blood 106:3970.
6. Frolova, E.G. et al. (2010) Circ. Res. 107:1313.
7. Sodersten, F. et al. (2007) Connect. Tiss. Res. 48:254.
8. Stenina, O.I. et al. (2003) Circulation 108:1514.
9. Arber, S. and P. Caroni (1995) J. Cell Biol. 131:1083.
10. Cingolani, O.H. et al. (2011) Circ. Res. 109:1410.
11. Stenina, O.I. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:1886.
12. Kim, D.-S. et al. (2012) J. Neurosci. 32:8977.
13. Dunkle, E.T. et al. (2007) Exp. Eye Res. 84:707.
14. Eroglu, C. et al. (2009) Cell 139:380.
15. Forster, S. et al. (2011) Mod. Pathol. 24:1390.

Publications for Thrombospondin-4 (7860-TH)(3)

Publications using 7860-TH

• K Imoto, M Aratani, T Koyama, M Okada, H Yamawaki Thrombospondin-4 induces prolongation of action potential duration in rat isolated ventricular myocytes J. Vet. Med. Sci., 2020-04-03;0(0):. 2020-04-03 [PMID: 32249254] (Cell Culture, Rat)
• TG Schips, D Vanhoutte, A Vo, RN Correll, MJ Brody, H Khalil, J Karch, A Tjondrokoe, MA Sargent, M Maillet, RS Ross, JD Molkentin Thrombospondin-3 augments injury-induced cardiomyopathy by intracellular integrin inhibition and sarcolemmal instability Nat Commun, 2019-01-08;10(1):76. 2019-01-08 [PMID: 30622267] (Bioassay, Mouse)
• Thrombospondin expression in myofibers stabilizes muscle membranes Elife, 2016-09-26;5(0):. 2016-09-26 [PMID: 27669143] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Thbs4
• Uniprot:
  • Q9Z1T2(Mouse)