Recombinant Mouse TACI/TNFRSF13B Fc Chimera Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse TACI/TNFRSF13B Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit BAFF-mediated proliferation of anti-IgM stimulated mouse B cells. Moore, P.A. et al. (1999) Science 285:260; Gross, J.A. et al. (2000) Nature 404:995; Schneider, P. et al. (1999) J. Exp. Med. 189:1747. The ED₅₀ for this effect is 3‑10 ng/mL in the presence of 2.5 ng/mL of rhBAFF.
• Source: Mouse myeloma cell line, NS0-derived mouse TACI/TNFRSF13B protein
  

  Mouse TACI (Phe5 - Thr129) Accession # Q9ET35	IEGRMD	Human IgG1 (Pro100 - Lys330)
  N-terminus		C-terminus

• Accession #: Q9ET35
• N-terminal Sequence: Phe5
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Tnfrsf13b
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 41 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 47 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse TACI/TNFRSF13B Fc Chimera Protein, CF

• CD267 antigen
• CD267
• CVID
• CVID2
• FLJ39942
• MGC133214
• TACI
• TACIMGC39952
• TNFRSF13B
• TNFRSF14B
• Transmembrane activator and CAML interactor
• tumor necrosis factor receptor 13B
• tumor necrosis factor receptor superfamily member 13B
• tumor necrosis factor receptor superfamily, member 13B

Background

TACI, transmembrane activator and CAML-interactor, is a member of the TNF receptor superfamily and has been designated TNFRSF13B. TACI is a type III membrane protein with an extracellular N-terminus in the absence of a cleaved signal sequence. The extracellular region of TACI contains two cysteine-rich domains. Within the TNFRSF, it shares the highest homology with B cell maturation factor (BCMA). TACI and BCMA have both been shown to bind APRIL and BAFF, members of the TNF ligand superfamily. TACI is expressed on the cell surface of B cells and activated, but not resting, T cells. Analogous to BCMA, data suggests that TACI may play an important role in B cell development, function and regulation. Mouse TACI is a 249 amino acid (aa) protein consisting of a 129 aa extracellular domain, a 20 aa transmembrane domain, and a 100 aa intracellular domain. Mouse and human TACI share 54% aa identity.

1. Xia, X.Z. et al. (2000) J. Exp. Med. 192:137.
2. von Bulow, G.U. et al. (1997) Science 278:138.
3. Gross, J.A. et al. (2000) Nature 404:995.
4. Marsters, S.A. et al. (2000) Curr. Biol. 10:785.
5. Yan, M. et al. (2000) Nature Immunol. 1:37.
6. Wu, Y. et al. (2000) J. Biol. Chem. 275:35478.

Publications for TACI/TNFRSF13B/CVID (1041-TC)(4)

Publications using 1041-TC

• Hoffmann F, Kuhn P, Laurent S, Hauck S, Berer K, Wendlinger S, Krumbholz M, Khademi M, Olsson T, Dreyling M, Pfister H, Alexander T, Hiepe F, Kumpfel T, Crawford H, Wekerle H, Hohlfeld R, Lichtenthaler S, Meinl E The immunoregulator soluble TACI is released by ADAM10 and reflects B cell activation in autoimmunity. J Immunol, 2014-12-10;194(2):542-52. 2014-12-10 [PMID: 25505277] (Bioassay, Mouse)
• Kotani Y, Kunisawa J, Suzuki Y, Sato I, Saito T, Toba M, Kohda N, Kiyono H Role of Lactobacillus pentosus Strain b240 and the Toll-like receptor 2 axis in Peyer&#039;s patch dendritic cell-mediated immunoglobulin A enhancement. PLoS ONE, 2014-03-14;9(3):e91857. 2014-03-14 [PMID: 24632732] (Bioassay, Mouse)
• Kimberley , Fiona C, van der Sloot , Almer M, Guadagnoli , Marco, Cameron , Katherin, Schneider , Pascal, Marquart , J Arnoud, Versloot , Miranda, Serrano , Luis, Medema , Jan Paul The Design and Characterization of Receptor-selective APRIL Variants. J Biol Chem, 2012-09-07;287(44):37434-46. 2012-09-07 [PMID: 22961987] (Binding Assay, Mouse)
• Suzuki K, Maruya M, Kawamoto S The sensing of environmental stimuli by follicular dendritic cells promotes immunoglobulin A generation in the gut. Immunity, 2010-07-23;33(1):71-83. 2010-07-23 [PMID: 20643338] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Tnfrsf13b
• Uniprot:
  • Q9ET35()