Recombinant Mouse Slit3 (aa 34-1116) Protein, CF

RecombinantMouse Slit3 (Catalog #9296-SL) Induces Cortical Neurite Outgrowth. A) Untreated E16-E18 embryonic rat corticalneurons. B) Neurite outgrowth in E16-E18embryonic rat cortical neurons treated with 5 μg/ml of Recombinant Mouse Slit3.

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Slit3 (aa 34-1116) Protein, CF Summary

• Details of Functionality: Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Recombinant Mouse Slit3, immobilized at 2.5-5 μg/mL on a 96-well plate, is able to significantly enhance neurite outgrowth.
• Source: Mouse myeloma cell line, NS0-derived mouse Slit3 protein
  Cys34-Val1116, with a C-terminal 6-His tag
• Accession #: Q9WVB4
• N-terminal Sequence: Cys34
• Protein/Peptide Type: Recombinant Proteins
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 122 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 121-153 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
• Buffer: Supplied as a 0.2 μm filtered solution in Sodium Acetate, NaCl and Ethylene Glycol.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Slit3 (aa 34-1116) Protein, CF

• KIAA0814
• MEGF5
• MEGF5Multiple epidermal growth factor-like domains protein 5
• Multiple EGF-like domains protein 5
• SLIL2
• SLIL2FLJ10764
• slit homolog 3 (Drosophila)
• slit homolog 3 protein
• SLIT1
• slit2
• Slit3
• Slit-3slit (Drosophila) homolog 3

Background

Slit3 is an approximately 200 kDa member of the Slit family of large secreted axon guidance molecules that are ligands for ROBO receptors (1, 2). It contains a mitochondrial targeting sequence and may also be retained intracellularly (3). Mature mouse Slit3 consists of 4 cassettes of leucine-rich repeats (LRR) flanked by LRR N-terminal and C-terminal domains, followed by multiple EGF-like domains, a Laminin G-like domain, and a C-terminal cysteine-rich domain (4). During development, Slit3 is expressed in the ventral neural tube, developing sensory organs, limb buds, and developing areas of the limbs in patterns that overlap with but are discrete from Slit1 and Slit2 (1, 2, 5). Axons will not be allowed to recross the floor plate unless all three Slit genes are disrupted, suggesting some overlap in Slit function (6). Slit3 is also expressed in the lung, kidney, skeletal muscle, and heart, both during development and postnatally (7-9). Mice with genetically disrupted Slit3 show abnormalities in diaphragm and kidney development (8, 9). Slit3 is additionally expressed by vascular endothelial cells and smooth muscle cells (10). It binds to both ROBO1 and ROBO4, but it is the ROBO4 interaction that mediates Slit3-induced angiogenesis (10). It also can enhance the chemokine-induced migration of monocytes (11) and inhibit tumor cell migration, invasion, and epithelial-mesenchymal transition (12, 13). The related Slit2 protein is cleaved in vivo (at a site conserved in Slit3), and the resulting C-terminal fragment binds Plexin A1 and retains the ability to repel axon migration (5, 14). The corresponding C-terminal fragment of Slit3 is able to bind heparin and neutralize its anti-coagulant activity (15). Within the N-terminal fragment (aa 34-1116), mouse Slit3 shares 95% and 98% amino acid identity with human and rat Slit3, respectively.

1. Blockus, H. and A. Chedotal (2014) Curr. Opin Neurobiol. 27:82.
2. Gara, R.K. et al. (2015) Drug Discov. Today 20:156.
3. Little, M. H. et al. (2001) Am. J. Physiol. Cell Physiol. 281:C486.
4. Yuan, W. et al. (1999) Dev. Biol. 212:290.
5. Brose, K. et al. (1999) Cell 96:795.
6. Long, H. et al. (2004) Neuron 42:213.
7. Greenberg, J.M. et al. (2004) Dev. Dyn. 230:350.
8. Liu, J. et al. (2003) Mech. Dev. 120:1059.
9. Yuan, W. et al. (2003) Proc. Natl. Acad. Sci. USA 100:5217.
10. Zhang, B. et al. (2009) Blood 114:4300.
11. Geutskens, S.B. et al. (2010) J. Immunol. 185:7691.
12. Denk, A.E. et al. (2011) Int. J. Mol. Med. 28:721.
13. Zhang, C. et al. (2015) Oncol. Rep. 34:952.
14. Delloye-Bourgeois, C. et al. (2015) Nat. Neurosci. 18:36.
15. Condac, E. et al. (2012) Glycobiology 22:1183.

Publications for Slit3 (9296-SL)(1)

Publication using 9296-SL

• B Mondal, H Jin, S Kallappago, Y Sedkov, T Martinez, MF Sentmanat, GJ Poet, C Li, Y Fan, SM Pruett-Mil, HM Herz The histone deacetylase complex MiDAC regulates a neurodevelopmental gene expression program to control neurite outgrowth Elife, 2020-04-16;9(0):. 2020-04-16 [PMID: 32297854] (Bioassay, Mouse)

Bioinformatics

• Uniprot:
  • Q9WVB4()