Recombinant Mouse Slit2 (aa 1114-1521) Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Slit2 (aa 1114-1521) Protein, CF Summary

• Details of Functionality: Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Recombinant Mouse Slit2, immobilized at 625 ng/mL on a 96 well plate, is able to significantly enhance neurite outgrowth.
• Source: Mouse myeloma cell line, NS0-derived mouse Slit2 protein
  Thr1114-Ser1521, with a C-terminal 6-His tag
• Accession #: Q9R1B9
• N-terminal Sequence: Thr1114
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 53-65 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in MOPS and NaCl.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Slit2 (aa 1114-1521) Protein, CF

• FLJ14420
• SLIL3
• slit (Drosophila) homolog 2
• slit homolog 2 (Drosophila)
• slit homolog 2 protein
• Slit2
• Slit-2SLIL3

Background

Slit Homolog 2 (Slit2) is a member of the Slit family of secreted extracellular matrix glycoproteins that are best known for their role in axon guidance (1). It is expressed in the developing and adult brain and spinal cord, as well as in fetal lung and kidney, and the adult adrenal gland, thyroid gland, and trachea (1-3). Slit2 is composed of multiple domains including nine EGF-like domains, 20 Leucine-rich repeats (LRRs), one Laminin G-like domain, one C-terminal cysteine knot-like (CTCK) domain, and 4 N-terminal and 4 C-terminal LRR domains (1, 3). Slit2 has a molecular weight of approximately 200 kDa (4). However, proteolytic cleavage between the fifth and sixth EGF-like domains produces a membrane-bound 140 kDa N-terminal protein, termed Slit2-N, and a 55-60 kDa C-terminal fragment, termed Slit2-C (4, 5). Mature mouse Slit2 shares 96% and 98% amino acid sequence identity with the human and rat orthologs, respectively.

Slit2 has been shown to bind to multiple receptors including ROBO-1, -2, -3, and -4, Laminin-1, DAN, Gremlin, and Glypican 1 (1, 6-8). Depending upon the target, Slit2 can promote a number of diverse effects. Slit2 regulates axon guidance by binding to ROBO receptors and initiating axon repulsion (1, 5, 9-12). Slit2 has also been shown to induce growth cone collapse, inhibit oligodendrocyte precursor cell migration, and promote axon elongation, branch formation, and fasciculation (5, 13-16). Additionally, Slit2-N and Slit2-C have been shown to have distinct activities. Slit2-N binds to ROBO-1 and repels motor axon migration, while Slit2-C binds to Glypican 1 and promotes motor axon migration (5). Outside the nervous system, C-terminal fragment of Slit-2 activates a thermogenic PKA pathway in adipocytes and improves glucose homeostasis (17).

1. Ypsilanti, A.R. et al. (2010) Development 137:1939.
2. Itoh, A. et al. (1998) Brain Res. Mol. Brain Res. 62:175.
3. Holmes, G.P. et al. (1998) Mech. Dev. 79:57.
4. Chédotal, A. (2007) Adv. Exp. Med. Biol. 621:65.
5. Nguyen Ba-Charvet, K.T. et al. (2001) J. Neurosci. 21:4281.
6. Nguyen Ba-Charvet, K.T. et al. (2001) Mol. Cell. Neurosci. 17:1048.
7. Hagino, S. et al. (2003) Glia 42:130.
8. Chen, B. et al. (2004) J. Immunol. 173:5914.
9. Yuan, W. et al. (1999) Dev. Biol. 212:290.
10. Erskine, L. et al. (2000) J. Neurosci. 20:4975.
11. Shu, T. et al. (2003) J. Neurosci. 23:8176.
12. Kim, M. et al. (2014) Neural Dev. 9:17.
13. Wang, K.H. et al. (1999) Cell 96:771.
14. Wong, E.V. et al. (2004) J. Neurobiol. 59:66.
15. Liu, X. et al. (2012) J. Biol. Chem. 287:17503.
16. Jaworski, A. et al. (2012) Nat. Neurosci. 15:367.
17. Svensson, K.J. et al. (2016) Cell Metabolism. 23:1.

Bioinformatics

• Uniprot:
  • Q9R1B9(Mouse)