Recombinant Mouse SLAM/CD150 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse SLAM/CD150 Protein, CF Summary

• Details of Functionality: Measured by its ability to co-stimulate IL-4 secretion by D10.G4.1 mouse helper T cells in the presence of anti-CD3. The ED₅₀ for this effect is 0.6-2.4 μg/mL.
  Optimal dilutions should be determined by each laboratory for each application.
• Source: Mouse myeloma cell line, NS0-derived mouse SLAM/CD150 protein
  Thr25-Pro242, with a C-terminal 6-His tag
• Accession #: Q9QUM4
• N-terminal Sequence: Thr25
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 25 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 41-55 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 200 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse SLAM/CD150 Protein, CF

• CD 150
• CD150
• CD150IPO-3
• CDw150
• IPO-3
• signaling lymphocytic activation molecule family member 1
• signaling lymphocytic activation molecule
• SLAM
• SLAMCD150 antigen
• SLAMF1

Background

The type I transmembrane glycoprotein Signaling Lymphocytic Activation Molecule (SLAM), also known as CD150, is the prototypic member of the SLAM subgroup of the CD2 protein family. CD2 family proteins function as adhesion molecules and modulators of the immune response (1). Mature mouse SLAM consists of a 218 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 23 aa transmembrane segment, and a 78 aa cytoplasmic domain with three immunoreceptor tyrosine switch motifs (ITSM) (2). Within the ECD, mouse SLAM shares 58% and 83% aa sequence identity with human and rat SLAM, respectively. Alternate splicing generates an isoform with a substituted cytoplasmic domain (2). It is expressed as a 75 kDa molecule of which approximately 30 kDa is N-linked carbohydrate (3). SLAM is expressed on T cells, B cells, thymocytes, macrophages, dendritic cells, platelets, and hematopoietic stem cells (2-7). It is up‑regulated on activated B cells and CD4⁺ and CD8⁺ T cells, although it is down‑regulated on Th2 polarized cells (2, 3, 8). SLAM interacts homophilically with low affinity, and this interaction induces a Th0/Th1 response characterized by clonal expansion, production of IFN-gamma , and increased cytolytic activity of CD8⁺ T cells (2, 3, 9‑11). SLAM ligation also promotes B cell activation, allergen-induced eosinophil and mast cell activation, NKT cell development, and the microbicidal response of macrophages to Gram negative bacteria (8, 12-14). In humans, SLAM functions as a cellular entry receptor for measles virus (15, 16).

1. Cannons, J.L. et al. (2011) Annu. Rev. Immunol. 29:665.
2. Castro, A.G. et al. (1999) J. Immunol. 163:5860.
3. Cocks, B.G. et al. (1995) Nature 376:260.
4. Wang, N. et al. (2004) J. Exp. Med. 199:1255.
5. Hahm, B. et al. (2004) Virology 323:292.
6. Nanda, N. et al. (2005) Blood 106:3028.
7. Kiel, M.J. et al. (2005) Cell 121:1109.
8. Punnonen, J. et al. (1997) J. Exp. Med. 185:993.
9. Mavaddat, N. et al. (2000) J. Biol. Chem. 275:28100.
10. Aversa, G. et al. (1997) J. Immunol. 158:4036.
11. Mehrle, S. et al. (2008) Mol. Immunol. 45:796.
12. Wang, N. et al. (2006) Am. J. Respir. Cell Mol. Biol. 35:206.
13. Jordan, M.A. et al. (2011) J. Immunol. 186:3953.
14. Berger, S.B. et al. (2010) Nat. Immunol. 11:920.
15. Tatsuo, H. et al. (2000) Nature 406:893.
16. Hsu, E.C. et al. (2001) Virology 279:9.

Bioinformatics

• Uniprot:
  • Q9QUM4()